Epithelial-mesenchymal transition-related gene expression as a new prognostic marker for neuroblastoma

Neuroblastoma (NB) is a highly metastatic tumor in children. The epithelial-mesenchymal transition (EMT) is an important mechanism for both the initiation of tumor invasion and subsequent metastasis. This study investigated the role of EMT in the progression of NB. Using EMT assays on samples from 1...

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Main Authors:	NOZATO, MEGUMI, KANEKO, SETSUKO, NAKAGAWARA, AKIRA, KOMURO, HIROAKI
Format:	Online
Language:	English
Published:	D.A. Spandidos 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583632/

id	pubmed-3583632
recordtype	oai_dc
spelling	pubmed-35836322013-03-04 Epithelial-mesenchymal transition-related gene expression as a new prognostic marker for neuroblastoma NOZATO, MEGUMI KANEKO, SETSUKO NAKAGAWARA, AKIRA KOMURO, HIROAKI Articles Neuroblastoma (NB) is a highly metastatic tumor in children. The epithelial-mesenchymal transition (EMT) is an important mechanism for both the initiation of tumor invasion and subsequent metastasis. This study investigated the role of EMT in the progression of NB. Using EMT assays on samples from 11 tumors, we identified 14 genes that were either differentially expressed between tumors of different stages or highly upregulated in NB. Quantitative RT-PCR of these genes was conducted in 96 NB tumors and their expression levels were compared between stages and between tumors with the presence and absence of MYCN amplification. The association of survival rate with differential gene expression was investigated. Expression of KRT19 was significantly decreased in stage 3 or 4 NB as well as stage 4S NB compared with stage 1 or 2 NB. Expression levels of KRT19 and ERBB3 were significantly low, and expression levels of TWST1 and TCF3 were high in MYCN-amplified NB. The patients with low expression of KRT19 or ERBB3 showed significantly worse overall survival. Furthermore, the correlation between high invasive ability and low expression of KRT19 and ERBB3 was suggested in vitro using six NB cell lines. The authors conclude that downregulation of KRT19 is highly associated with tumor progression in NB and metastasis in localized primary NB and that low expression of ERBB3 is also associated with progression of NB. D.A. Spandidos 2012-11-06 /pmc/articles/PMC3583632/ /pubmed/23135478 http://dx.doi.org/10.3892/ijo.2012.1684 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	NOZATO, MEGUMI KANEKO, SETSUKO NAKAGAWARA, AKIRA KOMURO, HIROAKI
spellingShingle	NOZATO, MEGUMI KANEKO, SETSUKO NAKAGAWARA, AKIRA KOMURO, HIROAKI Epithelial-mesenchymal transition-related gene expression as a new prognostic marker for neuroblastoma
author_facet	NOZATO, MEGUMI KANEKO, SETSUKO NAKAGAWARA, AKIRA KOMURO, HIROAKI
author_sort	NOZATO, MEGUMI
title	Epithelial-mesenchymal transition-related gene expression as a new prognostic marker for neuroblastoma
title_short	Epithelial-mesenchymal transition-related gene expression as a new prognostic marker for neuroblastoma
title_full	Epithelial-mesenchymal transition-related gene expression as a new prognostic marker for neuroblastoma
title_fullStr	Epithelial-mesenchymal transition-related gene expression as a new prognostic marker for neuroblastoma
title_full_unstemmed	Epithelial-mesenchymal transition-related gene expression as a new prognostic marker for neuroblastoma
title_sort	epithelial-mesenchymal transition-related gene expression as a new prognostic marker for neuroblastoma
description	Neuroblastoma (NB) is a highly metastatic tumor in children. The epithelial-mesenchymal transition (EMT) is an important mechanism for both the initiation of tumor invasion and subsequent metastasis. This study investigated the role of EMT in the progression of NB. Using EMT assays on samples from 11 tumors, we identified 14 genes that were either differentially expressed between tumors of different stages or highly upregulated in NB. Quantitative RT-PCR of these genes was conducted in 96 NB tumors and their expression levels were compared between stages and between tumors with the presence and absence of MYCN amplification. The association of survival rate with differential gene expression was investigated. Expression of KRT19 was significantly decreased in stage 3 or 4 NB as well as stage 4S NB compared with stage 1 or 2 NB. Expression levels of KRT19 and ERBB3 were significantly low, and expression levels of TWST1 and TCF3 were high in MYCN-amplified NB. The patients with low expression of KRT19 or ERBB3 showed significantly worse overall survival. Furthermore, the correlation between high invasive ability and low expression of KRT19 and ERBB3 was suggested in vitro using six NB cell lines. The authors conclude that downregulation of KRT19 is highly associated with tumor progression in NB and metastasis in localized primary NB and that low expression of ERBB3 is also associated with progression of NB.
publisher	D.A. Spandidos
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583632/
_version_	1611957976805933056

Epithelial-mesenchymal transition-related gene expression as a new prognostic marker for neuroblastoma

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