Establishment of a Lentiviral Vector Encoding Human HGF and the Infection of Human ADSCs

The delivery of adipose-derived stem cells (ADSCs) for promoting tissue repair has become a potential new therapy, while hepatocyte growth factor (HGF) is an important growth factor with angiogenic, anti-fibrotic, and anti-inflammatory benefits. In this paper, hADSCs were separated, cultured and ide...

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Main Authors:	Zhu, Xiaoyu, Xu, Lei, Liu, Xin, Wu, Jingsheng, Zhu, Weibo, Cai, Xiaoyan, Sun, Zimin
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581132/

id	pubmed-3581132
recordtype	oai_dc
spelling	pubmed-35811322013-03-12 Establishment of a Lentiviral Vector Encoding Human HGF and the Infection of Human ADSCs Zhu, Xiaoyu Xu, Lei Liu, Xin Wu, Jingsheng Zhu, Weibo Cai, Xiaoyan Sun, Zimin Research Article The delivery of adipose-derived stem cells (ADSCs) for promoting tissue repair has become a potential new therapy, while hepatocyte growth factor (HGF) is an important growth factor with angiogenic, anti-fibrotic, and anti-inflammatory benefits. In this paper, hADSCs were separated, cultured and identified based on the expression of cell surface antigens and multiple differentiation potential. We successfully generated a lentiviral vector encoding human HGF, infected hADSCs with this vector and examined the protein expression pattern. Finally we found that the hHGF lentiviral vector was successfully generated, and the lentiviral vector was able to safely infect hADSCs with high infection efficiency, thereby producing cells that overexpressed hHGF, which may provide a new strategy for the treatment of ischemic heart disease (IHD) and other ischemic diseases. Hindawi Publishing Corporation 2013 2013-01-20 /pmc/articles/PMC3581132/ /pubmed/23484149 http://dx.doi.org/10.1155/2013/724896 Text en Copyright © 2013 Xiaoyu Zhu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Zhu, Xiaoyu Xu, Lei Liu, Xin Wu, Jingsheng Zhu, Weibo Cai, Xiaoyan Sun, Zimin
spellingShingle	Zhu, Xiaoyu Xu, Lei Liu, Xin Wu, Jingsheng Zhu, Weibo Cai, Xiaoyan Sun, Zimin Establishment of a Lentiviral Vector Encoding Human HGF and the Infection of Human ADSCs
author_facet	Zhu, Xiaoyu Xu, Lei Liu, Xin Wu, Jingsheng Zhu, Weibo Cai, Xiaoyan Sun, Zimin
author_sort	Zhu, Xiaoyu
title	Establishment of a Lentiviral Vector Encoding Human HGF and the Infection of Human ADSCs
title_short	Establishment of a Lentiviral Vector Encoding Human HGF and the Infection of Human ADSCs
title_full	Establishment of a Lentiviral Vector Encoding Human HGF and the Infection of Human ADSCs
title_fullStr	Establishment of a Lentiviral Vector Encoding Human HGF and the Infection of Human ADSCs
title_full_unstemmed	Establishment of a Lentiviral Vector Encoding Human HGF and the Infection of Human ADSCs
title_sort	establishment of a lentiviral vector encoding human hgf and the infection of human adscs
description	The delivery of adipose-derived stem cells (ADSCs) for promoting tissue repair has become a potential new therapy, while hepatocyte growth factor (HGF) is an important growth factor with angiogenic, anti-fibrotic, and anti-inflammatory benefits. In this paper, hADSCs were separated, cultured and identified based on the expression of cell surface antigens and multiple differentiation potential. We successfully generated a lentiviral vector encoding human HGF, infected hADSCs with this vector and examined the protein expression pattern. Finally we found that the hHGF lentiviral vector was successfully generated, and the lentiviral vector was able to safely infect hADSCs with high infection efficiency, thereby producing cells that overexpressed hHGF, which may provide a new strategy for the treatment of ischemic heart disease (IHD) and other ischemic diseases.
publisher	Hindawi Publishing Corporation
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581132/
_version_	1611957303276208128

Establishment of a Lentiviral Vector Encoding Human HGF and the Infection of Human ADSCs

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