Roles and Mechanism of miR-199a and miR-125b in Tumor Angiogenesis

MicroRNAs (miRNAs) have been shown to be involved in different aspects of cancer biology including tumor angiogenesis. In this study, we identified that two miRNAs, miR-199a and miR-125b were downregulated in ovarian cancer tissues and cell lines. Overexpression of miR-199a and miR-125b inhibited tu...

Full description

Bibliographic Details
Main Authors:	He, Jun, Jing, Yi, Li, Wei, Qian, Xu, Xu, Qing, Li, Feng-Shan, Liu, Ling-Zhi, Jiang, Bing-Hua, Jiang, Yue
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577861/

id	pubmed-3577861
recordtype	oai_dc
spelling	pubmed-35778612013-02-22 Roles and Mechanism of miR-199a and miR-125b in Tumor Angiogenesis He, Jun Jing, Yi Li, Wei Qian, Xu Xu, Qing Li, Feng-Shan Liu, Ling-Zhi Jiang, Bing-Hua Jiang, Yue Research Article MicroRNAs (miRNAs) have been shown to be involved in different aspects of cancer biology including tumor angiogenesis. In this study, we identified that two miRNAs, miR-199a and miR-125b were downregulated in ovarian cancer tissues and cell lines. Overexpression of miR-199a and miR-125b inhibited tumor-induced angiogenesis associated with the decrease of HIF-1α and VEGF expression in ovarian cancer cells. Moreover, the levels of miR-199a and miR-125b were negatively correlated with VEGF mRNA levels in ovarian tissues. We further showed that direct targets of miR-199a and miR-125b HER2 and HER3 were functionally relevant. Forced expression of HER2 and HER3 rescued miR-199a- and miR-125b-inhibiting angiogenesis responses and Akt/p70S6K1/HIF-1α pathway. This study provides a rationale for new therapeutic approach to suppress tumor angiogenesis using miR-199a, miR-125b, or their mimics for ovarian cancer treatment in the future. Public Library of Science 2013-02-20 /pmc/articles/PMC3577861/ /pubmed/23437196 http://dx.doi.org/10.1371/journal.pone.0056647 Text en © 2013 He et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	He, Jun Jing, Yi Li, Wei Qian, Xu Xu, Qing Li, Feng-Shan Liu, Ling-Zhi Jiang, Bing-Hua Jiang, Yue
spellingShingle	He, Jun Jing, Yi Li, Wei Qian, Xu Xu, Qing Li, Feng-Shan Liu, Ling-Zhi Jiang, Bing-Hua Jiang, Yue Roles and Mechanism of miR-199a and miR-125b in Tumor Angiogenesis
author_facet	He, Jun Jing, Yi Li, Wei Qian, Xu Xu, Qing Li, Feng-Shan Liu, Ling-Zhi Jiang, Bing-Hua Jiang, Yue
author_sort	He, Jun
title	Roles and Mechanism of miR-199a and miR-125b in Tumor Angiogenesis
title_short	Roles and Mechanism of miR-199a and miR-125b in Tumor Angiogenesis
title_full	Roles and Mechanism of miR-199a and miR-125b in Tumor Angiogenesis
title_fullStr	Roles and Mechanism of miR-199a and miR-125b in Tumor Angiogenesis
title_full_unstemmed	Roles and Mechanism of miR-199a and miR-125b in Tumor Angiogenesis
title_sort	roles and mechanism of mir-199a and mir-125b in tumor angiogenesis
description	MicroRNAs (miRNAs) have been shown to be involved in different aspects of cancer biology including tumor angiogenesis. In this study, we identified that two miRNAs, miR-199a and miR-125b were downregulated in ovarian cancer tissues and cell lines. Overexpression of miR-199a and miR-125b inhibited tumor-induced angiogenesis associated with the decrease of HIF-1α and VEGF expression in ovarian cancer cells. Moreover, the levels of miR-199a and miR-125b were negatively correlated with VEGF mRNA levels in ovarian tissues. We further showed that direct targets of miR-199a and miR-125b HER2 and HER3 were functionally relevant. Forced expression of HER2 and HER3 rescued miR-199a- and miR-125b-inhibiting angiogenesis responses and Akt/p70S6K1/HIF-1α pathway. This study provides a rationale for new therapeutic approach to suppress tumor angiogenesis using miR-199a, miR-125b, or their mimics for ovarian cancer treatment in the future.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577861/
_version_	1611956427453104128

Roles and Mechanism of miR-199a and miR-125b in Tumor Angiogenesis

Similar Items