Disposable Fluorescence Optical pH Sensor for Near Neutral Solutions
The design, development and performance evaluation of a fluorescence-based pH sensor for on-line measurements is presented. The pKa of the sensing element has been calculated to be 7.9, thus the sensor is suitable for measurement of near neutral solutions. The sensor consists of a low-cost disposabl...
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| Format: | Online |
| Language: | English |
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MDPI
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574686/ |
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pubmed-35746862013-02-25 Disposable Fluorescence Optical pH Sensor for Near Neutral Solutions Ferrari, Luca Rovati, Luigi Fabbri, Paola Pilati, Francesco Article The design, development and performance evaluation of a fluorescence-based pH sensor for on-line measurements is presented. The pKa of the sensing element has been calculated to be 7.9, thus the sensor is suitable for measurement of near neutral solutions. The sensor consists of a low-cost disposable polymer sensing probe, in contact with the solution under test, interrogated by an optoelectronic transduction system. The pH sensitive dye is based on fluorescein O-methacrylate, which has been covalently linked to a hydrogel matrix, realized through the use of HEMA (2-hydroxyethyl methacrylate), HDDA (1,6-hexanediol diacrylate) and PEGDA (polyethylene glycol diacrylate). The optical interrogation setup, together with the electronics, has been developed to acquire and process the fluorescence signal. The sensor works over a pH range between 6.5 and 9.0. In the range between 7.0 and 8.0, the sensor shows a linear behavior with a maximum linearity error of 5%. Thanks to the good performance of the sensing element and transduction system, the short term drift of the reading (measured over 40 min) is lower than 0.15%. The measuring system also exhibits good performance in terms of response time and reproducibility. MDPI 2012-12-28 /pmc/articles/PMC3574686/ /pubmed/23275084 http://dx.doi.org/10.3390/s130100484 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Ferrari, Luca Rovati, Luigi Fabbri, Paola Pilati, Francesco |
| spellingShingle |
Ferrari, Luca Rovati, Luigi Fabbri, Paola Pilati, Francesco Disposable Fluorescence Optical pH Sensor for Near Neutral Solutions |
| author_facet |
Ferrari, Luca Rovati, Luigi Fabbri, Paola Pilati, Francesco |
| author_sort |
Ferrari, Luca |
| title |
Disposable Fluorescence Optical pH Sensor for Near Neutral Solutions |
| title_short |
Disposable Fluorescence Optical pH Sensor for Near Neutral Solutions |
| title_full |
Disposable Fluorescence Optical pH Sensor for Near Neutral Solutions |
| title_fullStr |
Disposable Fluorescence Optical pH Sensor for Near Neutral Solutions |
| title_full_unstemmed |
Disposable Fluorescence Optical pH Sensor for Near Neutral Solutions |
| title_sort |
disposable fluorescence optical ph sensor for near neutral solutions |
| description |
The design, development and performance evaluation of a fluorescence-based pH sensor for on-line measurements is presented. The pKa of the sensing element has been calculated to be 7.9, thus the sensor is suitable for measurement of near neutral solutions. The sensor consists of a low-cost disposable polymer sensing probe, in contact with the solution under test, interrogated by an optoelectronic transduction system. The pH sensitive dye is based on fluorescein O-methacrylate, which has been covalently linked to a hydrogel matrix, realized through the use of HEMA (2-hydroxyethyl methacrylate), HDDA (1,6-hexanediol diacrylate) and PEGDA (polyethylene glycol diacrylate). The optical interrogation setup, together with the electronics, has been developed to acquire and process the fluorescence signal. The sensor works over a pH range between 6.5 and 9.0. In the range between 7.0 and 8.0, the sensor shows a linear behavior with a maximum linearity error of 5%. Thanks to the good performance of the sensing element and transduction system, the short term drift of the reading (measured over 40 min) is lower than 0.15%. The measuring system also exhibits good performance in terms of response time and reproducibility. |
| publisher |
MDPI |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574686/ |
| _version_ |
1611955576683626496 |