Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells

Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells

In recent years, algae have been highlighted as potential sources of anticancer agents. Laminarin is a molecule found in marine brown algae that has potentially beneficial biological activities. However, these activities have not been investigated. In the present study, we examined the effects of la...

Full description

Bibliographic Details
Main Authors: PARK, HEE-KYOUNG, KIM, IN-HYE, KIM, JOONGKYUN, NAM, TAEK-JEONG
Format: Online
Language:English
Published: D.A. Spandidos 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573771/
id pubmed-3573771
recordtype oai_dc
spelling pubmed-35737712013-02-21 Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells PARK, HEE-KYOUNG KIM, IN-HYE KIM, JOONGKYUN NAM, TAEK-JEONG Articles In recent years, algae have been highlighted as potential sources of anticancer agents. Laminarin is a molecule found in marine brown algae that has potentially beneficial biological activities. However, these activities have not been investigated. In the present study, we examined the effects of laminarin on HT-29 cells and analyzed its effect on the insulin-like growth factor (IGF-IR) signaling pathway. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assays revealed that laminarin induced cell death in a dose-dependent manner. Western blotting showed that laminarin decreased mitogen-activated protein kinases (MAPK) and ERK phosphorylation. Decreased proliferation depended on IGF-IR, which was associated with the downregulation of MAPK/ERK. These results are important for understanding the roles of IGF-IR in colon cancer cell tumorigenesis, and suggest that laminarin shows activity against human colon cancer. D.A. Spandidos 2012-10 2012-08-02 /pmc/articles/PMC3573771/ /pubmed/22859258 http://dx.doi.org/10.3892/ijmm.2012.1084 Text en Copyright © 2012, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author PARK, HEE-KYOUNG
KIM, IN-HYE
KIM, JOONGKYUN
NAM, TAEK-JEONG
spellingShingle PARK, HEE-KYOUNG
KIM, IN-HYE
KIM, JOONGKYUN
NAM, TAEK-JEONG
Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells
author_facet PARK, HEE-KYOUNG
KIM, IN-HYE
KIM, JOONGKYUN
NAM, TAEK-JEONG
author_sort PARK, HEE-KYOUNG
title Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells
title_short Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells
title_full Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells
title_fullStr Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells
title_full_unstemmed Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells
title_sort induction of apoptosis by laminarin, regulating the insulin-like growth factor-ir signaling pathways in ht-29 human colon cells
description In recent years, algae have been highlighted as potential sources of anticancer agents. Laminarin is a molecule found in marine brown algae that has potentially beneficial biological activities. However, these activities have not been investigated. In the present study, we examined the effects of laminarin on HT-29 cells and analyzed its effect on the insulin-like growth factor (IGF-IR) signaling pathway. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assays revealed that laminarin induced cell death in a dose-dependent manner. Western blotting showed that laminarin decreased mitogen-activated protein kinases (MAPK) and ERK phosphorylation. Decreased proliferation depended on IGF-IR, which was associated with the downregulation of MAPK/ERK. These results are important for understanding the roles of IGF-IR in colon cancer cell tumorigenesis, and suggest that laminarin shows activity against human colon cancer.
publisher D.A. Spandidos
publishDate 2012
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573771/
_version_ 1611955308463128576

Similar Items