Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells

Our previous proteomic analysis revealed the expression of Rab28 in arteries of rats. However, the function of Rab28 in mammalian cells, and its role in vessels are still unknown. Coarctation of abdominal aorta above left kidney artery in rat was used as hypertensive animal model. FX-4000 cyclic str...

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Main Authors:	Jiang, Jun, Qi, Ying-Xin, Zhang, Ping, Gu, Wen-Tian, Yan, Zhi-Qiang, Shen, Bao-Rong, Yao, Qing-Ping, Kong, Han, Chien, Shu, Jiang, Zong-Lai
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573041/

id	pubmed-3573041
recordtype	oai_dc
spelling	pubmed-35730412013-03-01 Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells Jiang, Jun Qi, Ying-Xin Zhang, Ping Gu, Wen-Tian Yan, Zhi-Qiang Shen, Bao-Rong Yao, Qing-Ping Kong, Han Chien, Shu Jiang, Zong-Lai Research Article Our previous proteomic analysis revealed the expression of Rab28 in arteries of rats. However, the function of Rab28 in mammalian cells, and its role in vessels are still unknown. Coarctation of abdominal aorta above left kidney artery in rat was used as hypertensive animal model. FX-4000 cyclic strain loading system was used to mimic the mechanical condition on vascular cells during hypertension in vitro. Immunofluorescence and co-immunoprecipitation (Co-IP) were used to identify distribution and interaction of Rab28 and nuclear factor kappa B (NF-κB). Rab28 expression was significantly increased in carotid arteries of hypertensive rats. High cyclic strain induced Rab28 expression of endothelial cells (ECs) through a paracrine control of vascular smooth muscles cells (VSMCs), which at least partly via angiotensin II (Ang II). Rab28 knockdown decreased proliferation of ECs, while increased apoptosis and migration. Immunofluorescence revealed that Ang II stimulated the co-translocation of Rab28 and NF-κB from cytoplasm into nucleus. Knockdown of Rab28 attenuated NF-κB activation. Co-IP of NF-κB p65 and Rab28 indicated their interaction. Our results revealed that Rab28, as a novel regulator of NF-κB nuclear transport, might participate in the disturbance of EC homeostasis. Public Library of Science 2013-02-14 /pmc/articles/PMC3573041/ /pubmed/23457503 http://dx.doi.org/10.1371/journal.pone.0056076 Text en © 2013 Jiang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Jiang, Jun Qi, Ying-Xin Zhang, Ping Gu, Wen-Tian Yan, Zhi-Qiang Shen, Bao-Rong Yao, Qing-Ping Kong, Han Chien, Shu Jiang, Zong-Lai
spellingShingle	Jiang, Jun Qi, Ying-Xin Zhang, Ping Gu, Wen-Tian Yan, Zhi-Qiang Shen, Bao-Rong Yao, Qing-Ping Kong, Han Chien, Shu Jiang, Zong-Lai Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells
author_facet	Jiang, Jun Qi, Ying-Xin Zhang, Ping Gu, Wen-Tian Yan, Zhi-Qiang Shen, Bao-Rong Yao, Qing-Ping Kong, Han Chien, Shu Jiang, Zong-Lai
author_sort	Jiang, Jun
title	Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells
title_short	Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells
title_full	Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells
title_fullStr	Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells
title_full_unstemmed	Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells
title_sort	involvement of rab28 in nf-κb nuclear transport in endothelial cells
description	Our previous proteomic analysis revealed the expression of Rab28 in arteries of rats. However, the function of Rab28 in mammalian cells, and its role in vessels are still unknown. Coarctation of abdominal aorta above left kidney artery in rat was used as hypertensive animal model. FX-4000 cyclic strain loading system was used to mimic the mechanical condition on vascular cells during hypertension in vitro. Immunofluorescence and co-immunoprecipitation (Co-IP) were used to identify distribution and interaction of Rab28 and nuclear factor kappa B (NF-κB). Rab28 expression was significantly increased in carotid arteries of hypertensive rats. High cyclic strain induced Rab28 expression of endothelial cells (ECs) through a paracrine control of vascular smooth muscles cells (VSMCs), which at least partly via angiotensin II (Ang II). Rab28 knockdown decreased proliferation of ECs, while increased apoptosis and migration. Immunofluorescence revealed that Ang II stimulated the co-translocation of Rab28 and NF-κB from cytoplasm into nucleus. Knockdown of Rab28 attenuated NF-κB activation. Co-IP of NF-κB p65 and Rab28 indicated their interaction. Our results revealed that Rab28, as a novel regulator of NF-κB nuclear transport, might participate in the disturbance of EC homeostasis.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573041/
_version_	1611955062921232384

Involvement of Rab28 in NF-κB Nuclear Transport in Endothelial Cells

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