A subset of methylated CpG sites differentiate psoriatic from normal skin
Psoriasis is a chronic inflammatory immune-mediated disorder affecting the skin and other organs including joints. Over 1,300 transcripts are altered in psoriatic involved skin compared to normal skin. However to our knowledge global epigenetic profiling of psoriatic skin is previously unreported. H...
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2011
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3568942/ |
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pubmed-35689422013-02-11 A subset of methylated CpG sites differentiate psoriatic from normal skin Roberson, Elisha D.O. Liu, Ying Ryan, Caitriona Joyce, Cailin E. Duan, Shengui Cao, Li Martin, Ann Liao, Wilson Menter, Alan Bowcock, Anne M. Article Psoriasis is a chronic inflammatory immune-mediated disorder affecting the skin and other organs including joints. Over 1,300 transcripts are altered in psoriatic involved skin compared to normal skin. However to our knowledge global epigenetic profiling of psoriatic skin is previously unreported. Here we describe a genome-wide study of altered CpG methylation in psoriatic skin. We determined the methylation levels at 27,578 CpG sites in skin samples from individuals with psoriasis (12 involved, 8 uninvolved) and 10 unaffected individuals. CpG methylation of involved skin differed from normal skin at 1,108 sites. Twelve mapped to the epidermal differentiation complex, upstream or within genes that are highly up-regulated in psoriasis. Hierarchical clustering of 50 of the top differentially methylated (DM) sites separated psoriatic from normal skin samples. CpG sites where methylation was correlated with gene expression are reported. Sites with inverse correlations between methylation and nearby gene expression include those of KYNU, OAS2, S100A12, and SERPINB3, whose strong transcriptional up-regulation are important discriminators of psoriasis. We observed intrinsic epigenetic differences in uninvolved skin. Pyrosequencing of bisulfite-treated DNA from skin biopsies at three DM loci confirmed earlier findings and revealed reversion of methylation levels towards the non-psoriatic state after one month of anti-TNF-α therapy. 2011-11-10 2012-03 /pmc/articles/PMC3568942/ /pubmed/22071477 http://dx.doi.org/10.1038/jid.2011.348 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Roberson, Elisha D.O. Liu, Ying Ryan, Caitriona Joyce, Cailin E. Duan, Shengui Cao, Li Martin, Ann Liao, Wilson Menter, Alan Bowcock, Anne M. |
| spellingShingle |
Roberson, Elisha D.O. Liu, Ying Ryan, Caitriona Joyce, Cailin E. Duan, Shengui Cao, Li Martin, Ann Liao, Wilson Menter, Alan Bowcock, Anne M. A subset of methylated CpG sites differentiate psoriatic from normal skin |
| author_facet |
Roberson, Elisha D.O. Liu, Ying Ryan, Caitriona Joyce, Cailin E. Duan, Shengui Cao, Li Martin, Ann Liao, Wilson Menter, Alan Bowcock, Anne M. |
| author_sort |
Roberson, Elisha D.O. |
| title |
A subset of methylated CpG sites differentiate psoriatic from normal skin |
| title_short |
A subset of methylated CpG sites differentiate psoriatic from normal skin |
| title_full |
A subset of methylated CpG sites differentiate psoriatic from normal skin |
| title_fullStr |
A subset of methylated CpG sites differentiate psoriatic from normal skin |
| title_full_unstemmed |
A subset of methylated CpG sites differentiate psoriatic from normal skin |
| title_sort |
subset of methylated cpg sites differentiate psoriatic from normal skin |
| description |
Psoriasis is a chronic inflammatory immune-mediated disorder affecting the skin and other organs including joints. Over 1,300 transcripts are altered in psoriatic involved skin compared to normal skin. However to our knowledge global epigenetic profiling of psoriatic skin is previously unreported. Here we describe a genome-wide study of altered CpG methylation in psoriatic skin. We determined the methylation levels at 27,578 CpG sites in skin samples from individuals with psoriasis (12 involved, 8 uninvolved) and 10 unaffected individuals. CpG methylation of involved skin differed from normal skin at 1,108 sites. Twelve mapped to the epidermal differentiation complex, upstream or within genes that are highly up-regulated in psoriasis. Hierarchical clustering of 50 of the top differentially methylated (DM) sites separated psoriatic from normal skin samples. CpG sites where methylation was correlated with gene expression are reported. Sites with inverse correlations between methylation and nearby gene expression include those of KYNU, OAS2, S100A12, and SERPINB3, whose strong transcriptional up-regulation are important discriminators of psoriasis. We observed intrinsic epigenetic differences in uninvolved skin. Pyrosequencing of bisulfite-treated DNA from skin biopsies at three DM loci confirmed earlier findings and revealed reversion of methylation levels towards the non-psoriatic state after one month of anti-TNF-α therapy. |
| publishDate |
2011 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3568942/ |
| _version_ |
1611953763586670592 |