A simple method for simultaneous RP-HPLC determination of indolic compounds related to bacterial biosynthesis of indole-3-acetic acid
In this short technical report, we present a fast and simple procedure for sample preparation and a single-run Reversed Phase High Performance Liquid Chromatography (RP-HPLC) determination of seven indoles (indole-3-acetic acid, indole-3-acetamide, indole-3-acetonitrile, indole-3-ethanol, indole-3-l...
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| Format: | Online |
| Language: | English |
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Springer Netherlands
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3568474/ |
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pubmed-3568474 |
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pubmed-35684742013-02-14 A simple method for simultaneous RP-HPLC determination of indolic compounds related to bacterial biosynthesis of indole-3-acetic acid Szkop, Michał Bielawski, Wiesław Short Communication In this short technical report, we present a fast and simple procedure for sample preparation and a single-run Reversed Phase High Performance Liquid Chromatography (RP-HPLC) determination of seven indoles (indole-3-acetic acid, indole-3-acetamide, indole-3-acetonitrile, indole-3-ethanol, indole-3-lactic acid, tryptamine and tryptophan) in bacterial culture supernatants. The separation of the analytes, after a single centrifugal filtration clean-up step, was performed using a gradient elution on a symmetry C8 column followed by fluorimetric detection (λex = 280/λem = 350 nm). The calibration curves were linear for all of the studied compounds over the concentration range of 0.0625–125 μg mL−1 (r2 ≥ 0.998) and the limits of detection were below 0.015 μg mL−1. The applicability of the method was confirmed by analysis of Pseudomonasputida culture supernatants. Springer Netherlands 2012-10-31 2013-03 /pmc/articles/PMC3568474/ /pubmed/23111785 http://dx.doi.org/10.1007/s10482-012-9838-4 Text en © The Author(s) 2012 |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Szkop, Michał Bielawski, Wiesław |
| spellingShingle |
Szkop, Michał Bielawski, Wiesław A simple method for simultaneous RP-HPLC determination of indolic compounds related to bacterial biosynthesis of indole-3-acetic acid |
| author_facet |
Szkop, Michał Bielawski, Wiesław |
| author_sort |
Szkop, Michał |
| title |
A simple method for simultaneous RP-HPLC determination of indolic compounds related to bacterial biosynthesis of indole-3-acetic acid |
| title_short |
A simple method for simultaneous RP-HPLC determination of indolic compounds related to bacterial biosynthesis of indole-3-acetic acid |
| title_full |
A simple method for simultaneous RP-HPLC determination of indolic compounds related to bacterial biosynthesis of indole-3-acetic acid |
| title_fullStr |
A simple method for simultaneous RP-HPLC determination of indolic compounds related to bacterial biosynthesis of indole-3-acetic acid |
| title_full_unstemmed |
A simple method for simultaneous RP-HPLC determination of indolic compounds related to bacterial biosynthesis of indole-3-acetic acid |
| title_sort |
simple method for simultaneous rp-hplc determination of indolic compounds related to bacterial biosynthesis of indole-3-acetic acid |
| description |
In this short technical report, we present a fast and simple procedure for sample preparation and a single-run Reversed Phase High Performance Liquid Chromatography (RP-HPLC) determination of seven indoles (indole-3-acetic acid, indole-3-acetamide, indole-3-acetonitrile, indole-3-ethanol, indole-3-lactic acid, tryptamine and tryptophan) in bacterial culture supernatants. The separation of the analytes, after a single centrifugal filtration clean-up step, was performed using a gradient elution on a symmetry C8 column followed by fluorimetric detection (λex = 280/λem = 350 nm). The calibration curves were linear for all of the studied compounds over the concentration range of 0.0625–125 μg mL−1 (r2 ≥ 0.998) and the limits of detection were below 0.015 μg mL−1. The applicability of the method was confirmed by analysis of Pseudomonasputida culture supernatants. |
| publisher |
Springer Netherlands |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3568474/ |
| _version_ |
1611953675948785664 |