A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis

Alcohol-induced injury has become one of the major causes for liver cirrhosis. However, the molecular mechanisms of ethanol-induced injury are not fully understood. To this end, we performed a dynamic plasma membrane proteomic research on rat model. A rat model from hepatitis to liver cirrhosis was...

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Main Authors:	Jia, Xiaofang, Yin, Lin, Feng, Yanling, Peng, Xia, Ma, Fang, Yao, Yamin, Liu, Xiaoqian, Zhang, Zhiyong, Yuan, Zhenghong, Zhang, Lijun
Format:	Online
Language:	English
Published:	BioMed Central 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558348/

id	pubmed-3558348
recordtype	oai_dc
spelling	pubmed-35583482013-01-31 A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis Jia, Xiaofang Yin, Lin Feng, Yanling Peng, Xia Ma, Fang Yao, Yamin Liu, Xiaoqian Zhang, Zhiyong Yuan, Zhenghong Zhang, Lijun Research Alcohol-induced injury has become one of the major causes for liver cirrhosis. However, the molecular mechanisms of ethanol-induced injury are not fully understood. To this end, we performed a dynamic plasma membrane proteomic research on rat model. A rat model from hepatitis to liver cirrhosis was developed. Plasma membrane from liver tissue with liver fibrosis stage of 2 and 4 (S2 and S4) was purified by sucrose density gradient centrifugation. Its purification was verified by western blotting. Proteins from plasma membrane were separated by two-dimensional electrophoresis (2DE) and differentially expressed proteins were identified by tandem mass spectrometry. 16 consistent differentially expressed proteins from S2 to S4 were identified by mass spectrometry. The expression of differentially expressed proteins annexin A6 and annexin A3 were verified by western blotting, and annexin A3 was futher verified by immunohistochemistry. Our research suggests a possible mechanism by which ethanol alters protein expression to enhance the liver fibrosis progression. These differentially expressed proteins might be new drug targets for treating alcoholic liver cirrhosis. BioMed Central 2012-06-08 /pmc/articles/PMC3558348/ /pubmed/22682408 http://dx.doi.org/10.1186/1477-5956-10-39 Text en Copyright ©2012 Jia et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Jia, Xiaofang Yin, Lin Feng, Yanling Peng, Xia Ma, Fang Yao, Yamin Liu, Xiaoqian Zhang, Zhiyong Yuan, Zhenghong Zhang, Lijun
spellingShingle	Jia, Xiaofang Yin, Lin Feng, Yanling Peng, Xia Ma, Fang Yao, Yamin Liu, Xiaoqian Zhang, Zhiyong Yuan, Zhenghong Zhang, Lijun A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis
author_facet	Jia, Xiaofang Yin, Lin Feng, Yanling Peng, Xia Ma, Fang Yao, Yamin Liu, Xiaoqian Zhang, Zhiyong Yuan, Zhenghong Zhang, Lijun
author_sort	Jia, Xiaofang
title	A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis
title_short	A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis
title_full	A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis
title_fullStr	A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis
title_full_unstemmed	A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis
title_sort	dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis
description	Alcohol-induced injury has become one of the major causes for liver cirrhosis. However, the molecular mechanisms of ethanol-induced injury are not fully understood. To this end, we performed a dynamic plasma membrane proteomic research on rat model. A rat model from hepatitis to liver cirrhosis was developed. Plasma membrane from liver tissue with liver fibrosis stage of 2 and 4 (S2 and S4) was purified by sucrose density gradient centrifugation. Its purification was verified by western blotting. Proteins from plasma membrane were separated by two-dimensional electrophoresis (2DE) and differentially expressed proteins were identified by tandem mass spectrometry. 16 consistent differentially expressed proteins from S2 to S4 were identified by mass spectrometry. The expression of differentially expressed proteins annexin A6 and annexin A3 were verified by western blotting, and annexin A3 was futher verified by immunohistochemistry. Our research suggests a possible mechanism by which ethanol alters protein expression to enhance the liver fibrosis progression. These differentially expressed proteins might be new drug targets for treating alcoholic liver cirrhosis.
publisher	BioMed Central
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558348/
_version_	1611950545419894784

A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis

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