Expression and Purification of Functionally Active Recombinant Human Alpha 1-Antitrypsin in Methylotrophic Yeast Pichia pastoris

Expression and Purification of Functionally Active Recombinant Human Alpha 1-Antitrypsin in Methylotrophic Yeast Pichia pastoris

Human alpha 1-antitrypsin (AAT) cDNA was obtained from HepG2 cell lines. After PCR and construction of expression vector pPICZα-AAT, human AAT was expressed in the yeast Pichia pastoris (P.pastoris) in a secretary manner and under the control of inducible alcohol oxidase 1 (AOX1) promoter. The amoun...

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Bibliographic Details
Main Authors: Arjmand, Sareh, Bidram, Elham, Lotfi, Abbas Sahebghadam, Shamsara, Mehdi, Mowla, Seyed Javad
Format: Online
Language:English
Published: Avicenna Research Institute 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558188/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558188/

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