STED Nanoscopy with Time-Gated Detection: Theoretical and Experimental Aspects

In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event. This fact has been recently used to substantially improve the effective spatial resolution in STED nanoscopy...

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Bibliographic Details
Main Authors: Vicidomini, Giuseppe, Schönle, Andreas, Ta, Haisen, Han, Kyu Young, Moneron, Gael, Eggeling, Christian, Hell, Stefan W.
Format: Online
Language:English
Published: Public Library of Science 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3548795/