STED Nanoscopy with Time-Gated Detection: Theoretical and Experimental Aspects
In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event. This fact has been recently used to substantially improve the effective spatial resolution in STED nanoscopy...
Main Authors: | , , , , , , |
---|---|
Format: | Online |
Language: | English |
Published: |
Public Library of Science
2013
|
Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3548795/ |