Targeted proteomics reveals compositional dynamics of 60S pre-ribosomes after nuclear export

A combination of genetic trapping, affinity-capture and selected reaction monitoring mass spectrometry is used to characterize the dynamic proteome of pre-60S ribosomal particles after nuclear export. These results identify Bud20 as a novel shuttling factor for pre-60S export.

Bibliographic Details
Main Authors: Altvater, Martin, Chang, Yiming, Melnik, Andre, Occhipinti, Laura, Schütz, Sabina, Rothenbusch, Ute, Picotti, Paola, Panse, Vikram Govind
Format: Online
Language:English
Published: Nature Publishing Group 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542530/