Condensation of an Additive-Free Cell Extract to Mimic the Conditions of Live Cells

The cellular environment differs from that of reconstituted materials mainly because of the presence of highly condensed biomacromolecules. To mimic the environment and conditions in living cells, we developed a method to prepare additive-free, highly concentrated cell extracts. First, we verified t...

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Bibliographic Details
Main Authors: Fujiwara, Kei, Nomura, Shin-ichiro M.
Format: Online
Language:English
Published: Public Library of Science 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3542322/