Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice

Reactive α,β-unsaturated aldehydes such as acrolein (ACR) are major components of environmental pollutants and have been implicated in the neurodegenerative and cardiac diseases. In this study, the protective effect of silymarin (SN) against cardiotoxicity induced by ACR in mice was evaluated. Studi...

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Main Authors:	Taghiabadi, Elahe, Imenshahidi, Mohsen, Abnous, Khalil, Mosafa, Fatemeh, Sankian, Mojtaba, Memar, Bahram, Karimi, Gholamreza
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3535759/

id	pubmed-3535759
recordtype	oai_dc
spelling	pubmed-35357592013-01-14 Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice Taghiabadi, Elahe Imenshahidi, Mohsen Abnous, Khalil Mosafa, Fatemeh Sankian, Mojtaba Memar, Bahram Karimi, Gholamreza Research Article Reactive α,β-unsaturated aldehydes such as acrolein (ACR) are major components of environmental pollutants and have been implicated in the neurodegenerative and cardiac diseases. In this study, the protective effect of silymarin (SN) against cardiotoxicity induced by ACR in mice was evaluated. Studies were performed on seven groups of six animals each, including vehicle-control (normal saline + 0.5% w/v methylcellulose), ACR (7.5 mg/kg/day, gavage) for 3 weeks, SN (25, 50 and 100 mg/kg/day, i.p.) plus ACR, vitamin E (Vit E, 100 IU/kg, i.p.) plus ACR, and SN (100 mg/kg, i.p.) groups. Mice received SN 7 days before ACR and daily thereafter throughout the study. Pretreatment with SN attenuated ACR-induced increased levels of malondialdehyde (MDA), serum cardiac troponin I (cTnI), and creatine kinase-MB (CK-MB), as well as histopathological changes in cardiac tissues. Moreover, SN improved glutathione (GSH) content, superoxide dismutase (SOD), and catalase (CAT) activities in heart of ACR-treated mice. Western blot analysis showed that SN pretreatment inhibited apoptosis provoked by ACR through decreasing Bax/Bcl-2 ratio, cytosolic cytochrome c content, and cleaved caspase-3 level in heart. In conclusion, SN may have protective effects against cardiotoxicity of ACR by reducing lipid peroxidation, renewing the activities of antioxidant enzymes, and preventing apoptosis. Hindawi Publishing Corporation 2012 2012-12-18 /pmc/articles/PMC3535759/ /pubmed/23320028 http://dx.doi.org/10.1155/2012/352091 Text en Copyright © 2012 Elahe Taghiabadi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Taghiabadi, Elahe Imenshahidi, Mohsen Abnous, Khalil Mosafa, Fatemeh Sankian, Mojtaba Memar, Bahram Karimi, Gholamreza
spellingShingle	Taghiabadi, Elahe Imenshahidi, Mohsen Abnous, Khalil Mosafa, Fatemeh Sankian, Mojtaba Memar, Bahram Karimi, Gholamreza Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice
author_facet	Taghiabadi, Elahe Imenshahidi, Mohsen Abnous, Khalil Mosafa, Fatemeh Sankian, Mojtaba Memar, Bahram Karimi, Gholamreza
author_sort	Taghiabadi, Elahe
title	Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice
title_short	Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice
title_full	Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice
title_fullStr	Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice
title_full_unstemmed	Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice
title_sort	protective effect of silymarin against acrolein-induced cardiotoxicity in mice
description	Reactive α,β-unsaturated aldehydes such as acrolein (ACR) are major components of environmental pollutants and have been implicated in the neurodegenerative and cardiac diseases. In this study, the protective effect of silymarin (SN) against cardiotoxicity induced by ACR in mice was evaluated. Studies were performed on seven groups of six animals each, including vehicle-control (normal saline + 0.5% w/v methylcellulose), ACR (7.5 mg/kg/day, gavage) for 3 weeks, SN (25, 50 and 100 mg/kg/day, i.p.) plus ACR, vitamin E (Vit E, 100 IU/kg, i.p.) plus ACR, and SN (100 mg/kg, i.p.) groups. Mice received SN 7 days before ACR and daily thereafter throughout the study. Pretreatment with SN attenuated ACR-induced increased levels of malondialdehyde (MDA), serum cardiac troponin I (cTnI), and creatine kinase-MB (CK-MB), as well as histopathological changes in cardiac tissues. Moreover, SN improved glutathione (GSH) content, superoxide dismutase (SOD), and catalase (CAT) activities in heart of ACR-treated mice. Western blot analysis showed that SN pretreatment inhibited apoptosis provoked by ACR through decreasing Bax/Bcl-2 ratio, cytosolic cytochrome c content, and cleaved caspase-3 level in heart. In conclusion, SN may have protective effects against cardiotoxicity of ACR by reducing lipid peroxidation, renewing the activities of antioxidant enzymes, and preventing apoptosis.
publisher	Hindawi Publishing Corporation
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3535759/
_version_	1611944345263407104

Protective Effect of Silymarin against Acrolein-Induced Cardiotoxicity in Mice

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