Molecular Parentage Analysis Is Essential in Breeding Asian Seabass

In aquaculture species, maintaining pedigree information and genetic variation in each generation is essential, but very difficult. In this study, we used nine microsatellites to genotype 2,520 offspring from four independent full-factorial crosses (10 males ×10 females) of Asian seabass to reconstr...

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Main Authors:	Liu, Peng, Xia, Jun Hong, Lin, Grace, Sun, Fei, Liu, Feng, Lim, Huan Sein, Pang, Hong Yan, Yue, Gen Hua
Format:	Online
Language:	English
Published:	Public Library of Science 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3515488/

id	pubmed-3515488
recordtype	oai_dc
spelling	pubmed-35154882012-12-07 Molecular Parentage Analysis Is Essential in Breeding Asian Seabass Liu, Peng Xia, Jun Hong Lin, Grace Sun, Fei Liu, Feng Lim, Huan Sein Pang, Hong Yan Yue, Gen Hua Research Article In aquaculture species, maintaining pedigree information and genetic variation in each generation is essential, but very difficult. In this study, we used nine microsatellites to genotype 2,520 offspring from four independent full-factorial crosses (10 males ×10 females) of Asian seabass to reconstruct pedigree and monitor the change of genetic variations. In all four crosses, over 96.8% of the offspring could be assigned to their parents, indicating the high power of the nine microsatellites for parentage assignment. This study revealed several interesting results: (1). In all four crosses, the contribution of parents to offspring was significantly uneven, and some dominant breeding fishes (i.e. brooders) were found; (2). In two mass crosses where the brooders were carefully checked for reproductive status, a majority (≥90%) of brooders contributed to offspring, whereas in another two crosses, where the brooders were randomly picked without checking reproductive status, only a few brooders (40.0–45.0%) produced offspring; (3). Females had more problems in successful spawning compared to males; and (4). In the two crosses where a few brooders produced offspring, there was a substantial loss in allelic (24.1–34.3%) and gene (20.5–25.7%) diversities in offspring, while in the other two crosses, the majority of allelic (96.8–97.0%) and gene diversities (94.8–97.1%) were maintained. These observations suggest that a routine molecular parentage analysis is required to maintain both allelic and gene diversity in breeding Asian seabass. Public Library of Science 2012-12-05 /pmc/articles/PMC3515488/ /pubmed/23227245 http://dx.doi.org/10.1371/journal.pone.0051142 Text en © 2012 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Liu, Peng Xia, Jun Hong Lin, Grace Sun, Fei Liu, Feng Lim, Huan Sein Pang, Hong Yan Yue, Gen Hua
spellingShingle	Liu, Peng Xia, Jun Hong Lin, Grace Sun, Fei Liu, Feng Lim, Huan Sein Pang, Hong Yan Yue, Gen Hua Molecular Parentage Analysis Is Essential in Breeding Asian Seabass
author_facet	Liu, Peng Xia, Jun Hong Lin, Grace Sun, Fei Liu, Feng Lim, Huan Sein Pang, Hong Yan Yue, Gen Hua
author_sort	Liu, Peng
title	Molecular Parentage Analysis Is Essential in Breeding Asian Seabass
title_short	Molecular Parentage Analysis Is Essential in Breeding Asian Seabass
title_full	Molecular Parentage Analysis Is Essential in Breeding Asian Seabass
title_fullStr	Molecular Parentage Analysis Is Essential in Breeding Asian Seabass
title_full_unstemmed	Molecular Parentage Analysis Is Essential in Breeding Asian Seabass
title_sort	molecular parentage analysis is essential in breeding asian seabass
description	In aquaculture species, maintaining pedigree information and genetic variation in each generation is essential, but very difficult. In this study, we used nine microsatellites to genotype 2,520 offspring from four independent full-factorial crosses (10 males ×10 females) of Asian seabass to reconstruct pedigree and monitor the change of genetic variations. In all four crosses, over 96.8% of the offspring could be assigned to their parents, indicating the high power of the nine microsatellites for parentage assignment. This study revealed several interesting results: (1). In all four crosses, the contribution of parents to offspring was significantly uneven, and some dominant breeding fishes (i.e. brooders) were found; (2). In two mass crosses where the brooders were carefully checked for reproductive status, a majority (≥90%) of brooders contributed to offspring, whereas in another two crosses, where the brooders were randomly picked without checking reproductive status, only a few brooders (40.0–45.0%) produced offspring; (3). Females had more problems in successful spawning compared to males; and (4). In the two crosses where a few brooders produced offspring, there was a substantial loss in allelic (24.1–34.3%) and gene (20.5–25.7%) diversities in offspring, while in the other two crosses, the majority of allelic (96.8–97.0%) and gene diversities (94.8–97.1%) were maintained. These observations suggest that a routine molecular parentage analysis is required to maintain both allelic and gene diversity in breeding Asian seabass.
publisher	Public Library of Science
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3515488/
_version_	1611938430859608064

Molecular Parentage Analysis Is Essential in Breeding Asian Seabass

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