A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS)
The oral adsorbent AST-120 is composed of spherical carbon particles and has an adsorption ability for certain small-molecular-weight compounds that accumulate in patients with chronic kidney disease (CKD). So far, very few compounds are known to be adsorbed by AST-120 in vivo. To examine the effect...
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| Format: | Online |
| Language: | English |
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MDPI
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509710/ |
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pubmed-3509710 |
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pubmed-35097102012-12-10 A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS) Akiyama, Yasutoshi Takeuchi, Yoichi Kikuchi, Koichi Mishima, Eikan Yamamoto, Yasuaki Suzuki, Chitose Toyohara, Takafumi Suzuki, Takehiro Hozawa, Atsushi Ito, Sadayoshi Soga, Tomoyoshi Abe, Takaaki Article The oral adsorbent AST-120 is composed of spherical carbon particles and has an adsorption ability for certain small-molecular-weight compounds that accumulate in patients with chronic kidney disease (CKD). So far, very few compounds are known to be adsorbed by AST-120 in vivo. To examine the effect of AST-120 in vivo, we comprehensively evaluated the plasma concentrations of 146 compounds (61 anions and 85 cations) in CKD model rats, with or without four weeks of treatment with AST-120. By capillary electrophoresis with mass spectrometry, we identified 6 anions and 17 cations that were significantly decreased by AST-120 treatment. In contrast, we also identified 2 cations that were significantly increased by AST-120. Among them, 4 anions, apart from indoxyl sulfate and hippurate, and 19 cations were newly identified in this study. The plasma levels of N-acetyl-neuraminate, 4-pyridoxate, 4-oxopentanoate, glycine, γ-guanidinobutyrate, N-γ-ethylglutamine, allantoin, cytosine, 5-methylcytosine and imidazole-4-acetate were significantly increased in the CKD model compared with the sham-operated group, and were significantly decreased by AST-120 treatment. Therefore, these 10 compounds could be added as uremic compounds that indicate the effect of AST-120 treatment. This study provides useful information not only for identifying the indicators of AST-120, but also for clarifying changes in the metabolic profile by AST-120 treatment in the clinical setting. MDPI 2012-11-14 /pmc/articles/PMC3509710/ /pubmed/23202318 http://dx.doi.org/10.3390/toxins4111309 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Akiyama, Yasutoshi Takeuchi, Yoichi Kikuchi, Koichi Mishima, Eikan Yamamoto, Yasuaki Suzuki, Chitose Toyohara, Takafumi Suzuki, Takehiro Hozawa, Atsushi Ito, Sadayoshi Soga, Tomoyoshi Abe, Takaaki |
| spellingShingle |
Akiyama, Yasutoshi Takeuchi, Yoichi Kikuchi, Koichi Mishima, Eikan Yamamoto, Yasuaki Suzuki, Chitose Toyohara, Takafumi Suzuki, Takehiro Hozawa, Atsushi Ito, Sadayoshi Soga, Tomoyoshi Abe, Takaaki A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS) |
| author_facet |
Akiyama, Yasutoshi Takeuchi, Yoichi Kikuchi, Koichi Mishima, Eikan Yamamoto, Yasuaki Suzuki, Chitose Toyohara, Takafumi Suzuki, Takehiro Hozawa, Atsushi Ito, Sadayoshi Soga, Tomoyoshi Abe, Takaaki |
| author_sort |
Akiyama, Yasutoshi |
| title |
A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS) |
| title_short |
A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS) |
| title_full |
A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS) |
| title_fullStr |
A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS) |
| title_full_unstemmed |
A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS) |
| title_sort |
metabolomic approach to clarifying the effect of ast-120 on 5/6 nephrectomized rats by capillary electrophoresis with mass spectrometry (ce-ms) |
| description |
The oral adsorbent AST-120 is composed of spherical carbon particles and has an adsorption ability for certain small-molecular-weight compounds that accumulate in patients with chronic kidney disease (CKD). So far, very few compounds are known to be adsorbed by AST-120 in vivo. To examine the effect of AST-120 in vivo, we comprehensively evaluated the plasma concentrations of 146 compounds (61 anions and 85 cations) in CKD model rats, with or without four weeks of treatment with AST-120. By capillary electrophoresis with mass spectrometry, we identified 6 anions and 17 cations that were significantly decreased by AST-120 treatment. In contrast, we also identified 2 cations that were significantly increased by AST-120. Among them, 4 anions, apart from indoxyl sulfate and hippurate, and 19 cations were newly identified in this study. The plasma levels of N-acetyl-neuraminate, 4-pyridoxate, 4-oxopentanoate, glycine, γ-guanidinobutyrate, N-γ-ethylglutamine, allantoin, cytosine, 5-methylcytosine and imidazole-4-acetate were significantly increased in the CKD model compared with the sham-operated group, and were significantly decreased by AST-120 treatment. Therefore, these 10 compounds could be added as uremic compounds that indicate the effect of AST-120 treatment. This study provides useful information not only for identifying the indicators of AST-120, but also for clarifying changes in the metabolic profile by AST-120 treatment in the clinical setting. |
| publisher |
MDPI |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509710/ |
| _version_ |
1611936502620618752 |