Phase-contrast X-ray microtomography of mouse fetus

A phase-contrast X-ray microtomography system using the Talbot imaging has been built at the SPring-8 synchrotron radiation facility. This system has much higher density resolution than absorption-based X-ray microtomography. The tomographic sections of formalin-fixed mouse fetuses obtained with thi...

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Main Authors: Hoshino, Masato, Uesugi, Kentaro, Yagi, Naoto
Format: Online
Language:English
Published: The Company of Biologists 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507284/
id pubmed-3507284
recordtype oai_dc
spelling pubmed-35072842012-12-04 Phase-contrast X-ray microtomography of mouse fetus Hoshino, Masato Uesugi, Kentaro Yagi, Naoto Research Article A phase-contrast X-ray microtomography system using the Talbot imaging has been built at the SPring-8 synchrotron radiation facility. This system has much higher density resolution than absorption-based X-ray microtomography. The tomographic sections of formalin-fixed mouse fetuses obtained with this method clearly depict various organs without any staining at a pixel resolution of up to 5 µm. Since this technique allows us to obtain three-dimensional structural information without sectioning, it will be particularly useful to examine anomalies that take place during development. It can be also used to quantitatively measure volume and mass of organs during development. The Company of Biologists 2012-02-10 /pmc/articles/PMC3507284/ /pubmed/23213417 http://dx.doi.org/10.1242/bio.2012430 Text en © 2012. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by-nc-sa/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Hoshino, Masato
Uesugi, Kentaro
Yagi, Naoto
spellingShingle Hoshino, Masato
Uesugi, Kentaro
Yagi, Naoto
Phase-contrast X-ray microtomography of mouse fetus
author_facet Hoshino, Masato
Uesugi, Kentaro
Yagi, Naoto
author_sort Hoshino, Masato
title Phase-contrast X-ray microtomography of mouse fetus
title_short Phase-contrast X-ray microtomography of mouse fetus
title_full Phase-contrast X-ray microtomography of mouse fetus
title_fullStr Phase-contrast X-ray microtomography of mouse fetus
title_full_unstemmed Phase-contrast X-ray microtomography of mouse fetus
title_sort phase-contrast x-ray microtomography of mouse fetus
description A phase-contrast X-ray microtomography system using the Talbot imaging has been built at the SPring-8 synchrotron radiation facility. This system has much higher density resolution than absorption-based X-ray microtomography. The tomographic sections of formalin-fixed mouse fetuses obtained with this method clearly depict various organs without any staining at a pixel resolution of up to 5 µm. Since this technique allows us to obtain three-dimensional structural information without sectioning, it will be particularly useful to examine anomalies that take place during development. It can be also used to quantitatively measure volume and mass of organs during development.
publisher The Company of Biologists
publishDate 2012
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507284/
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