Development of bioartificial myocardium by electrostimulation of 3D collagen scaffolds seeded with stem cells

Electrostimulation (ES) can be defined as a safe physical method to induce stem cell differentiation. The aim of this study is to evaluate the effectiveness of ES on bone marrow mesenchymal stem cells (BMSCs) seeded in collagen scaffolds in terms of proliferation and differentiation into cardiomyocy...

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Main Authors:	Haneef, Kanwal, Lila, Nermine, Benadda, Samira, Legrand, Fabien, Carpentier, Alain, Chachques, Juan C.
Format:	Online
Language:	English
Published:	PAGEPress Publications 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3504306/

id	pubmed-3504306
recordtype	oai_dc
spelling	pubmed-35043062012-11-26 Development of bioartificial myocardium by electrostimulation of 3D collagen scaffolds seeded with stem cells Haneef, Kanwal Lila, Nermine Benadda, Samira Legrand, Fabien Carpentier, Alain Chachques, Juan C. Article Electrostimulation (ES) can be defined as a safe physical method to induce stem cell differentiation. The aim of this study is to evaluate the effectiveness of ES on bone marrow mesenchymal stem cells (BMSCs) seeded in collagen scaffolds in terms of proliferation and differentiation into cardiomyocytes. BMSCs were isolated from Wistar rats and seeded into 3D collagen type 1 templates measuring 25 × 25 × 6 mm. Bipolar in vitro ES was performed during 21 days. Electrical impedance and cell proliferation were measured. Expression of cardiac markers was assessed by immunocytochemistry. Viscoelasticity of collagen matrix was evaluated. Electrical impedance assessments showed a low resistance of 234±41 Ohms which indicates good electrical conductivity of collagen matrix. Cell proliferation at 570 nm as significantly increased in ES groups after seven day (ES 0.129±0.03 vs non-stimulated control matrix 0.06±0.01, P=0.002) and after 21 days, (ES 0.22±0.04 vs control 0.13±0.01, P=0.01). Immunocytoche mistry of BMSCs after 21 days ES showed positive staining of cardiac markers, troponin I, connexin 43, sarcomeric alpha-actinin, slow myosin, fast myosin and desmin. Staining for BMSCs marker CD29 after 21 days was negative. Electrostimulation of cell-seeded collagen matrix changed stem cell morphology and biochemical characteristics, increasing the expression of cardiac markers. Thus, MSC-derived differentiated cells by electrostimulation grafted in biological scaffolds might result in a convenient tissue engineering source for myocardial diseases. PAGEPress Publications 2012-09-18 /pmc/articles/PMC3504306/ /pubmed/23185681 http://dx.doi.org/10.4081/hi.2012.e14 Text en ©Copyright G. Argenti and G. Lombardi, 2012 This work is licensed under a Creative Commons Attribution NonCommercial 3.0 License (CC BY-NC 3.0). Licensee PAGEPress, Italy
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Haneef, Kanwal Lila, Nermine Benadda, Samira Legrand, Fabien Carpentier, Alain Chachques, Juan C.
spellingShingle	Haneef, Kanwal Lila, Nermine Benadda, Samira Legrand, Fabien Carpentier, Alain Chachques, Juan C. Development of bioartificial myocardium by electrostimulation of 3D collagen scaffolds seeded with stem cells
author_facet	Haneef, Kanwal Lila, Nermine Benadda, Samira Legrand, Fabien Carpentier, Alain Chachques, Juan C.
author_sort	Haneef, Kanwal
title	Development of bioartificial myocardium by electrostimulation of 3D collagen scaffolds seeded with stem cells
title_short	Development of bioartificial myocardium by electrostimulation of 3D collagen scaffolds seeded with stem cells
title_full	Development of bioartificial myocardium by electrostimulation of 3D collagen scaffolds seeded with stem cells
title_fullStr	Development of bioartificial myocardium by electrostimulation of 3D collagen scaffolds seeded with stem cells
title_full_unstemmed	Development of bioartificial myocardium by electrostimulation of 3D collagen scaffolds seeded with stem cells
title_sort	development of bioartificial myocardium by electrostimulation of 3d collagen scaffolds seeded with stem cells
description	Electrostimulation (ES) can be defined as a safe physical method to induce stem cell differentiation. The aim of this study is to evaluate the effectiveness of ES on bone marrow mesenchymal stem cells (BMSCs) seeded in collagen scaffolds in terms of proliferation and differentiation into cardiomyocytes. BMSCs were isolated from Wistar rats and seeded into 3D collagen type 1 templates measuring 25 × 25 × 6 mm. Bipolar in vitro ES was performed during 21 days. Electrical impedance and cell proliferation were measured. Expression of cardiac markers was assessed by immunocytochemistry. Viscoelasticity of collagen matrix was evaluated. Electrical impedance assessments showed a low resistance of 234±41 Ohms which indicates good electrical conductivity of collagen matrix. Cell proliferation at 570 nm as significantly increased in ES groups after seven day (ES 0.129±0.03 vs non-stimulated control matrix 0.06±0.01, P=0.002) and after 21 days, (ES 0.22±0.04 vs control 0.13±0.01, P=0.01). Immunocytoche mistry of BMSCs after 21 days ES showed positive staining of cardiac markers, troponin I, connexin 43, sarcomeric alpha-actinin, slow myosin, fast myosin and desmin. Staining for BMSCs marker CD29 after 21 days was negative. Electrostimulation of cell-seeded collagen matrix changed stem cell morphology and biochemical characteristics, increasing the expression of cardiac markers. Thus, MSC-derived differentiated cells by electrostimulation grafted in biological scaffolds might result in a convenient tissue engineering source for myocardial diseases.
publisher	PAGEPress Publications
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3504306/
_version_	1611934653191553024

Development of bioartificial myocardium by electrostimulation of 3D collagen scaffolds seeded with stem cells

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