Schistosoma japonicum Soluble Egg Antigens Attenuate IFN-γ-Induced MHC Class II Expression in RAW 264.7 Macrophages

Innate immune response plays the key role in initiating and guiding the immune response. Elucidating the innate immune related molecular events involved in the interaction between the parasite and the host will aid in the development of an effective vaccine and anti-schistosome pharmaceuticals. In t...

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Main Authors:	Tang, Gui-Xia, Zhou, He-Jun, Xu, Jin-Wei, Xu, Jin-Mei, Ji, Min-Jun, Wu, Hai-Wei, Wu, Guan-Ling
Format:	Online
Language:	English
Published:	Public Library of Science 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3496699/

id	pubmed-3496699
recordtype	oai_dc
spelling	pubmed-34966992012-11-14 Schistosoma japonicum Soluble Egg Antigens Attenuate IFN-γ-Induced MHC Class II Expression in RAW 264.7 Macrophages Tang, Gui-Xia Zhou, He-Jun Xu, Jin-Wei Xu, Jin-Mei Ji, Min-Jun Wu, Hai-Wei Wu, Guan-Ling Research Article Innate immune response plays the key role in initiating and guiding the immune response. Elucidating the innate immune related molecular events involved in the interaction between the parasite and the host will aid in the development of an effective vaccine and anti-schistosome pharmaceuticals. In this study, we examined the regulatory effect of Schistosoma japonicum soluble egg antigen (SEA) on MHC class II expression in macrophage cell line RAW 264.7. We demonstrated that SEA possesses the ability to down-regulate IFN-γ-induced MHC class II expression in RAW 264.7 cells. The production of IL-10 and IL-6 in RAW 264.7 cells, induced by SEA, was responsible for mediating the down-regulation of MHC class II. Our findings suggest that in RAW 264.7 cells (1) IFN-γ provides a condition for lower concentrations of SEA to attenuate MHC class II expression; (2) SEA attenuated IFN-γ-induced MHC class II expression and the IL-10 and IL-6 production is mediated at least partly by the interaction of SEA with TLR4; and (3) SEA attenuated IFN-γ-induced MHC class II expression at the transcriptional level. Public Library of Science 2012-11-13 /pmc/articles/PMC3496699/ /pubmed/23152879 http://dx.doi.org/10.1371/journal.pone.0049234 Text en © 2012 Tang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Tang, Gui-Xia Zhou, He-Jun Xu, Jin-Wei Xu, Jin-Mei Ji, Min-Jun Wu, Hai-Wei Wu, Guan-Ling
spellingShingle	Tang, Gui-Xia Zhou, He-Jun Xu, Jin-Wei Xu, Jin-Mei Ji, Min-Jun Wu, Hai-Wei Wu, Guan-Ling Schistosoma japonicum Soluble Egg Antigens Attenuate IFN-γ-Induced MHC Class II Expression in RAW 264.7 Macrophages
author_facet	Tang, Gui-Xia Zhou, He-Jun Xu, Jin-Wei Xu, Jin-Mei Ji, Min-Jun Wu, Hai-Wei Wu, Guan-Ling
author_sort	Tang, Gui-Xia
title	Schistosoma japonicum Soluble Egg Antigens Attenuate IFN-γ-Induced MHC Class II Expression in RAW 264.7 Macrophages
title_short	Schistosoma japonicum Soluble Egg Antigens Attenuate IFN-γ-Induced MHC Class II Expression in RAW 264.7 Macrophages
title_full	Schistosoma japonicum Soluble Egg Antigens Attenuate IFN-γ-Induced MHC Class II Expression in RAW 264.7 Macrophages
title_fullStr	Schistosoma japonicum Soluble Egg Antigens Attenuate IFN-γ-Induced MHC Class II Expression in RAW 264.7 Macrophages
title_full_unstemmed	Schistosoma japonicum Soluble Egg Antigens Attenuate IFN-γ-Induced MHC Class II Expression in RAW 264.7 Macrophages
title_sort	schistosoma japonicum soluble egg antigens attenuate ifn-γ-induced mhc class ii expression in raw 264.7 macrophages
description	Innate immune response plays the key role in initiating and guiding the immune response. Elucidating the innate immune related molecular events involved in the interaction between the parasite and the host will aid in the development of an effective vaccine and anti-schistosome pharmaceuticals. In this study, we examined the regulatory effect of Schistosoma japonicum soluble egg antigen (SEA) on MHC class II expression in macrophage cell line RAW 264.7. We demonstrated that SEA possesses the ability to down-regulate IFN-γ-induced MHC class II expression in RAW 264.7 cells. The production of IL-10 and IL-6 in RAW 264.7 cells, induced by SEA, was responsible for mediating the down-regulation of MHC class II. Our findings suggest that in RAW 264.7 cells (1) IFN-γ provides a condition for lower concentrations of SEA to attenuate MHC class II expression; (2) SEA attenuated IFN-γ-induced MHC class II expression and the IL-10 and IL-6 production is mediated at least partly by the interaction of SEA with TLR4; and (3) SEA attenuated IFN-γ-induced MHC class II expression at the transcriptional level.
publisher	Public Library of Science
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3496699/
_version_	1611923822096678912

Schistosoma japonicum Soluble Egg Antigens Attenuate IFN-γ-Induced MHC Class II Expression in RAW 264.7 Macrophages

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