Augmentation of transgenic expression by ultrasound-mediated liposome microbubble destruction

Non-invasive, efficient and tissue-specific transgenic technologies could be valuable in gene therapy. Although non-viral carriers may be safer and cheaper, they have a much lower transfection efficiency than viral gene carriers. The present study was designed to test the transgenic expression and s...

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Main Authors:	CHEN, ZHI-YI, SUN, XIAO-FANG, LIU, JIAN-QIAO, SI-TU, BING, QIU, RI-XIANG, LIANG, KUN, LIU, JIAN-HUA, LIANG, WEI-XIANG, ZHOU, XIN-XIN, ZHANG, HUA, YU, JIANG-XIU
Format:	Online
Language:	English
Published:	D.A. Spandidos 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493077/

id	pubmed-3493077
recordtype	oai_dc
spelling	pubmed-34930772013-04-01 Augmentation of transgenic expression by ultrasound-mediated liposome microbubble destruction CHEN, ZHI-YI SUN, XIAO-FANG LIU, JIAN-QIAO SI-TU, BING QIU, RI-XIANG LIANG, KUN LIU, JIAN-HUA LIANG, WEI-XIANG ZHOU, XIN-XIN ZHANG, HUA YU, JIANG-XIU Article Non-invasive, efficient and tissue-specific transgenic technologies could be valuable in gene therapy. Although non-viral carriers may be safer and cheaper, they have a much lower transfection efficiency than viral gene carriers. The present study was designed to test the transgenic expression and safety of red fluorescent protein (RFP) in HeLa cells in vitro and in transplanted tumors of nude mice in vivo under ultrasound-mediated liposome microbubble destruction (UMLMD) conditions. Plasmids containing RFP were gently mixed with liposome microbubbles (LMs). The mixture was added to HeLa cells or injected into BALB/c mice by the tail vein under various ultrasound exposure and LM parameters, and then the transfection efficiencies were examined. The results in vivo and in vitro demonstrated that, following a comparison of the plasmid group, the ultrasound + plasmid group and the LM + plasmid group, UMLMD significantly increased the transgenic expression (P<0.01) without causing any apparent detrimental effect. From the study, we concluded that UMLMD could be a non-invasive, effective and promising non-viral technique for gene therapy and transgenic research. D.A. Spandidos 2012-01-25 2012-04 /pmc/articles/PMC3493077/ /pubmed/22294278 http://dx.doi.org/10.3892/mmr.2012.766 Text en Copyright © 2012, Spandidos Publications
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	CHEN, ZHI-YI SUN, XIAO-FANG LIU, JIAN-QIAO SI-TU, BING QIU, RI-XIANG LIANG, KUN LIU, JIAN-HUA LIANG, WEI-XIANG ZHOU, XIN-XIN ZHANG, HUA YU, JIANG-XIU
spellingShingle	CHEN, ZHI-YI SUN, XIAO-FANG LIU, JIAN-QIAO SI-TU, BING QIU, RI-XIANG LIANG, KUN LIU, JIAN-HUA LIANG, WEI-XIANG ZHOU, XIN-XIN ZHANG, HUA YU, JIANG-XIU Augmentation of transgenic expression by ultrasound-mediated liposome microbubble destruction
author_facet	CHEN, ZHI-YI SUN, XIAO-FANG LIU, JIAN-QIAO SI-TU, BING QIU, RI-XIANG LIANG, KUN LIU, JIAN-HUA LIANG, WEI-XIANG ZHOU, XIN-XIN ZHANG, HUA YU, JIANG-XIU
author_sort	CHEN, ZHI-YI
title	Augmentation of transgenic expression by ultrasound-mediated liposome microbubble destruction
title_short	Augmentation of transgenic expression by ultrasound-mediated liposome microbubble destruction
title_full	Augmentation of transgenic expression by ultrasound-mediated liposome microbubble destruction
title_fullStr	Augmentation of transgenic expression by ultrasound-mediated liposome microbubble destruction
title_full_unstemmed	Augmentation of transgenic expression by ultrasound-mediated liposome microbubble destruction
title_sort	augmentation of transgenic expression by ultrasound-mediated liposome microbubble destruction
description	Non-invasive, efficient and tissue-specific transgenic technologies could be valuable in gene therapy. Although non-viral carriers may be safer and cheaper, they have a much lower transfection efficiency than viral gene carriers. The present study was designed to test the transgenic expression and safety of red fluorescent protein (RFP) in HeLa cells in vitro and in transplanted tumors of nude mice in vivo under ultrasound-mediated liposome microbubble destruction (UMLMD) conditions. Plasmids containing RFP were gently mixed with liposome microbubbles (LMs). The mixture was added to HeLa cells or injected into BALB/c mice by the tail vein under various ultrasound exposure and LM parameters, and then the transfection efficiencies were examined. The results in vivo and in vitro demonstrated that, following a comparison of the plasmid group, the ultrasound + plasmid group and the LM + plasmid group, UMLMD significantly increased the transgenic expression (P<0.01) without causing any apparent detrimental effect. From the study, we concluded that UMLMD could be a non-invasive, effective and promising non-viral technique for gene therapy and transgenic research.
publisher	D.A. Spandidos
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493077/
_version_	1611922670591410176

Augmentation of transgenic expression by ultrasound-mediated liposome microbubble destruction

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