The Single T65S Mutation Generates Brighter Cyan Fluorescent Proteins with Increased Photostability and pH Insensitivity

The Single T65S Mutation Generates Brighter Cyan Fluorescent Proteins with Increased Photostability and pH Insensitivity

Cyan fluorescent proteins (CFP) derived from Aequorea victoria GFP, carrying a tryptophan-based chromophore, are widely used as FRET donors in live cell fluorescence imaging experiments. Recently, several CFP variants with near-ultimate photophysical performances were obtained through a mix of site-...

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Bibliographic Details
Main Authors: Fredj, Asma, Pasquier, Hélène, Demachy, Isabelle, Jonasson, Gabriella, Levy, Bernard, Derrien, Valérie, Bousmah, Yasmina, Manoussaris, Gallia, Wien, Frank, Ridard, Jacqueline, Erard, Marie, Merola, Fabienne
Format: Online
Language:English
Published: Public Library of Science 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487735/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487735/

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