The Single T65S Mutation Generates Brighter Cyan Fluorescent Proteins with Increased Photostability and pH Insensitivity

Cyan fluorescent proteins (CFP) derived from Aequorea victoria GFP, carrying a tryptophan-based chromophore, are widely used as FRET donors in live cell fluorescence imaging experiments. Recently, several CFP variants with near-ultimate photophysical performances were obtained through a mix of site-...

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Bibliographic Details
Main Authors: Fredj, Asma, Pasquier, Hélène, Demachy, Isabelle, Jonasson, Gabriella, Levy, Bernard, Derrien, Valérie, Bousmah, Yasmina, Manoussaris, Gallia, Wien, Frank, Ridard, Jacqueline, Erard, Marie, Merola, Fabienne
Format: Online
Language:English
Published: Public Library of Science 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487735/