In Vitro Propagation of Muña-Muña (Clinopodium odorum (Griseb.) Harley)

In Vitro Propagation of Muña-Muña (Clinopodium odorum (Griseb.) Harley)

A micropropagation protocol was developed which may assist in the safeguarding and augmentation of dwindling natural populations of Clinopodium odorum (Griseb.) Harley, a critically and endangered medicinal plant. Factors affecting culture initiation bud sprouting and growth, rooting, and acclimatiz...

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Main Authors: Diaz, María Soledad, Palacio, Lorena, Figueroa, Ana Cristina, Goleniowski, Marta Ester
Format: Online
Language:English
Published: Hindawi Publishing Corporation 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483647/
id pubmed-3483647
recordtype oai_dc
spelling pubmed-34836472012-11-01 In Vitro Propagation of Muña-Muña (Clinopodium odorum (Griseb.) Harley) Diaz, María Soledad Palacio, Lorena Figueroa, Ana Cristina Goleniowski, Marta Ester Research Article A micropropagation protocol was developed which may assist in the safeguarding and augmentation of dwindling natural populations of Clinopodium odorum (Griseb.) Harley, a critically and endangered medicinal plant. Factors affecting culture initiation bud sprouting and growth, rooting, and acclimatization were studied, using nodal segments of in vitro germinated seedling as primary explants on six media supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP) (0.5–1.5 and 2-Naphthalene acetic acid (NAA) (0.5–1.5). Best results for culture initiation with sustainable multiplication rates (100%) were obtained on WP medium without any growth regulator. WP with the addition of 0.5 : 1 or 0.5 : 1.5) of BAP and NAA promoted a higher elongation; however, the optimum number of nodes were obtained in plantlets grown on 1/2 MS with the addition of 1 : 1.5 of BAP and NAA. Culture of sectioned individual nodes transferred to the media with different rates of BAP and NAA 1/2 MS-9 (1.5 : 1.5), SH-8 (1.5 : 1.0), and 1/2 B5-4 (1.0 : 0.5) media resulted in no proliferated shoots. The in vitro plants were successfully acclimatized garden soil and sand (2 : 1) in the greenhouse, with over 90% survival rate. The in vitro-grown plants could be transferred to ex vitro conditions and the efficacy in supporting ex vitro growth was assessed, with a view to develope longer-term strategies for the transfer and reintroduction into natural habitats. Hindawi Publishing Corporation 2012 2012-10-18 /pmc/articles/PMC3483647/ /pubmed/23119167 http://dx.doi.org/10.1155/2012/196583 Text en Copyright © 2012 María Soledad Diaz et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Diaz, María Soledad
Palacio, Lorena
Figueroa, Ana Cristina
Goleniowski, Marta Ester
spellingShingle Diaz, María Soledad
Palacio, Lorena
Figueroa, Ana Cristina
Goleniowski, Marta Ester
In Vitro Propagation of Muña-Muña (Clinopodium odorum (Griseb.) Harley)
author_facet Diaz, María Soledad
Palacio, Lorena
Figueroa, Ana Cristina
Goleniowski, Marta Ester
author_sort Diaz, María Soledad
title In Vitro Propagation of Muña-Muña (Clinopodium odorum (Griseb.) Harley)
title_short In Vitro Propagation of Muña-Muña (Clinopodium odorum (Griseb.) Harley)
title_full In Vitro Propagation of Muña-Muña (Clinopodium odorum (Griseb.) Harley)
title_fullStr In Vitro Propagation of Muña-Muña (Clinopodium odorum (Griseb.) Harley)
title_full_unstemmed In Vitro Propagation of Muña-Muña (Clinopodium odorum (Griseb.) Harley)
title_sort in vitro propagation of muña-muña (clinopodium odorum (griseb.) harley)
description A micropropagation protocol was developed which may assist in the safeguarding and augmentation of dwindling natural populations of Clinopodium odorum (Griseb.) Harley, a critically and endangered medicinal plant. Factors affecting culture initiation bud sprouting and growth, rooting, and acclimatization were studied, using nodal segments of in vitro germinated seedling as primary explants on six media supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP) (0.5–1.5 and 2-Naphthalene acetic acid (NAA) (0.5–1.5). Best results for culture initiation with sustainable multiplication rates (100%) were obtained on WP medium without any growth regulator. WP with the addition of 0.5 : 1 or 0.5 : 1.5) of BAP and NAA promoted a higher elongation; however, the optimum number of nodes were obtained in plantlets grown on 1/2 MS with the addition of 1 : 1.5 of BAP and NAA. Culture of sectioned individual nodes transferred to the media with different rates of BAP and NAA 1/2 MS-9 (1.5 : 1.5), SH-8 (1.5 : 1.0), and 1/2 B5-4 (1.0 : 0.5) media resulted in no proliferated shoots. The in vitro plants were successfully acclimatized garden soil and sand (2 : 1) in the greenhouse, with over 90% survival rate. The in vitro-grown plants could be transferred to ex vitro conditions and the efficacy in supporting ex vitro growth was assessed, with a view to develope longer-term strategies for the transfer and reintroduction into natural habitats.
publisher Hindawi Publishing Corporation
publishDate 2012
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483647/
_version_ 1611919727991455744

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