Establishment of glass catfish (Kryptopterus bicirrhis) fin-derived cells

Genetically manipulated transparent animals were already explored in many species for in vivo study of gene expression, transplantation analysis and cancer biology. However, there are no reports about transparent animals as in vitro genetic resources. In the present study, fin-derived cells from gla...

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Main Authors:	Han, Jee Eun, Choresca, Casiano H, Koo, Ok Jae, Oh, Hyun Ju, Hong, So Gun, Kim, Ji Hyung, Shin, Sang Phil, Jun, Jin Woo, Lee, Byeong Chun, Park, Se Chang
Format:	Online
Language:	English
Published:	Portland Press Ltd 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475438/

id	pubmed-3475438
recordtype	oai_dc
spelling	pubmed-34754382012-10-30 Establishment of glass catfish (Kryptopterus bicirrhis) fin-derived cells Han, Jee Eun Choresca, Casiano H Koo, Ok Jae Oh, Hyun Ju Hong, So Gun Kim, Ji Hyung Shin, Sang Phil Jun, Jin Woo Lee, Byeong Chun Park, Se Chang Research Article Genetically manipulated transparent animals were already explored in many species for in vivo study of gene expression, transplantation analysis and cancer biology. However, there are no reports about transparent animals as in vitro genetic resources. In the present study, fin-derived cells from glass catfish (Krytopterus bicirrhis), naturally transparent fish with a visible skeleton and internal organs, were isolated after culturing fin explants and characterized using cryopreservation and cell cycle analysis. The cells grew well in DMEM (Dulbecco's modified Eagle's medium) containing 1% (v/v) P/S (penicillin–streptomycin) and 10% (v/v) fetal bovine serum at 26°C and showed increased cryopreservation efficiency with the slow-freezing method in the presence of 15% dimethyl sulfoxide. In addition, cell cycle analysis was evaluated based on flow cytometric analysis, and culturing to confluence (>85%) was more effective for synchronizing cells at the G0/G1 stages than roscovitine treatment (<75%). This is the first report about cell isolation from transparent animals. The results from testing the cell's viability following cryopreservation and subjecting the cells to cycle analysis can be useful tools for genetic resource management. Portland Press Ltd 2011-03-18 /pmc/articles/PMC3475438/ /pubmed/23119145 http://dx.doi.org/10.1042/CBR20110002 Text en © The Author(s). http://creativecommons.org/licenses/by-nc/2.5/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commerical use, distribution and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Han, Jee Eun Choresca, Casiano H Koo, Ok Jae Oh, Hyun Ju Hong, So Gun Kim, Ji Hyung Shin, Sang Phil Jun, Jin Woo Lee, Byeong Chun Park, Se Chang
spellingShingle	Han, Jee Eun Choresca, Casiano H Koo, Ok Jae Oh, Hyun Ju Hong, So Gun Kim, Ji Hyung Shin, Sang Phil Jun, Jin Woo Lee, Byeong Chun Park, Se Chang Establishment of glass catfish (Kryptopterus bicirrhis) fin-derived cells
author_facet	Han, Jee Eun Choresca, Casiano H Koo, Ok Jae Oh, Hyun Ju Hong, So Gun Kim, Ji Hyung Shin, Sang Phil Jun, Jin Woo Lee, Byeong Chun Park, Se Chang
author_sort	Han, Jee Eun
title	Establishment of glass catfish (Kryptopterus bicirrhis) fin-derived cells
title_short	Establishment of glass catfish (Kryptopterus bicirrhis) fin-derived cells
title_full	Establishment of glass catfish (Kryptopterus bicirrhis) fin-derived cells
title_fullStr	Establishment of glass catfish (Kryptopterus bicirrhis) fin-derived cells
title_full_unstemmed	Establishment of glass catfish (Kryptopterus bicirrhis) fin-derived cells
title_sort	establishment of glass catfish (kryptopterus bicirrhis) fin-derived cells
description	Genetically manipulated transparent animals were already explored in many species for in vivo study of gene expression, transplantation analysis and cancer biology. However, there are no reports about transparent animals as in vitro genetic resources. In the present study, fin-derived cells from glass catfish (Krytopterus bicirrhis), naturally transparent fish with a visible skeleton and internal organs, were isolated after culturing fin explants and characterized using cryopreservation and cell cycle analysis. The cells grew well in DMEM (Dulbecco's modified Eagle's medium) containing 1% (v/v) P/S (penicillin–streptomycin) and 10% (v/v) fetal bovine serum at 26°C and showed increased cryopreservation efficiency with the slow-freezing method in the presence of 15% dimethyl sulfoxide. In addition, cell cycle analysis was evaluated based on flow cytometric analysis, and culturing to confluence (>85%) was more effective for synchronizing cells at the G0/G1 stages than roscovitine treatment (<75%). This is the first report about cell isolation from transparent animals. The results from testing the cell's viability following cryopreservation and subjecting the cells to cycle analysis can be useful tools for genetic resource management.
publisher	Portland Press Ltd
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475438/
_version_	1611917310283481088

Establishment of glass catfish (Kryptopterus bicirrhis) fin-derived cells

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