Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector

The molecular mechanisms of neuronal morphology and synaptic vesicle transport have been largely elusive, and only a few of the molecules involved in these processes have been identified. Here, we developed a novel morphology-based gene trap method, which is theoretically applicable to all cell line...

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Main Authors:	Hashimoto, Yukiko, Muramatsu, Kazuhiro, Kunii, Masataka, Yoshimura, Shin-ichiro, Yamada, Minami, Sato, Takashi, Ishida, Yasumasa, Harada, Reiko, Harada, Akihiro
Format:	Online
Language:	English
Published:	Federation of American Societies for Experimental Biology 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475256/

id	pubmed-3475256
recordtype	oai_dc
spelling	pubmed-34752562012-11-01 Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector Hashimoto, Yukiko Muramatsu, Kazuhiro Kunii, Masataka Yoshimura, Shin-ichiro Yamada, Minami Sato, Takashi Ishida, Yasumasa Harada, Reiko Harada, Akihiro Research Communications The molecular mechanisms of neuronal morphology and synaptic vesicle transport have been largely elusive, and only a few of the molecules involved in these processes have been identified. Here, we developed a novel morphology-based gene trap method, which is theoretically applicable to all cell lines, to easily and rapidly identify the responsible genes. Using this method, we selected several gene-trapped clones of rat pheochromocytoma PC12 cells, which displayed abnormal morphology and distribution of synaptic vesicle-like microvesicles (SLMVs). We identified several genes responsible for the phenotypes and analyzed three genes in more detail. The first gene was BTB/POZ domain-containing protein 9 (Btbd9), which is associated with restless legs syndrome. The second gene was cytokine receptor-like factor 3 (Crlf3), whose involvement in the nervous system remains unknown. The third gene was single-stranded DNA-binding protein 3 (Ssbp3), a gene known to regulate head morphogenesis. These results suggest that Btbd9, Crlf3, and Ssbp3 regulate neuronal morphology and the biogenesis/transport of synaptic vesicles. Because our novel morphology-based gene trap method is generally applicable, this method is promising for uncovering novel genes involved in the function of interest in any cell lines.—Hashimoto, Y., Muramatsu, K., Kunii, M., Yoshimura, S., Yamada, M., Sato, T., Ishida, Y., Harada, R., Harada, A. Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector. Federation of American Societies for Experimental Biology 2012-11 /pmc/articles/PMC3475256/ /pubmed/22874834 http://dx.doi.org/10.1096/fj.12-207530 Text en © FASEB This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/us/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Hashimoto, Yukiko Muramatsu, Kazuhiro Kunii, Masataka Yoshimura, Shin-ichiro Yamada, Minami Sato, Takashi Ishida, Yasumasa Harada, Reiko Harada, Akihiro
spellingShingle	Hashimoto, Yukiko Muramatsu, Kazuhiro Kunii, Masataka Yoshimura, Shin-ichiro Yamada, Minami Sato, Takashi Ishida, Yasumasa Harada, Reiko Harada, Akihiro Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector
author_facet	Hashimoto, Yukiko Muramatsu, Kazuhiro Kunii, Masataka Yoshimura, Shin-ichiro Yamada, Minami Sato, Takashi Ishida, Yasumasa Harada, Reiko Harada, Akihiro
author_sort	Hashimoto, Yukiko
title	Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector
title_short	Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector
title_full	Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector
title_fullStr	Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector
title_full_unstemmed	Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector
title_sort	uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector
description	The molecular mechanisms of neuronal morphology and synaptic vesicle transport have been largely elusive, and only a few of the molecules involved in these processes have been identified. Here, we developed a novel morphology-based gene trap method, which is theoretically applicable to all cell lines, to easily and rapidly identify the responsible genes. Using this method, we selected several gene-trapped clones of rat pheochromocytoma PC12 cells, which displayed abnormal morphology and distribution of synaptic vesicle-like microvesicles (SLMVs). We identified several genes responsible for the phenotypes and analyzed three genes in more detail. The first gene was BTB/POZ domain-containing protein 9 (Btbd9), which is associated with restless legs syndrome. The second gene was cytokine receptor-like factor 3 (Crlf3), whose involvement in the nervous system remains unknown. The third gene was single-stranded DNA-binding protein 3 (Ssbp3), a gene known to regulate head morphogenesis. These results suggest that Btbd9, Crlf3, and Ssbp3 regulate neuronal morphology and the biogenesis/transport of synaptic vesicles. Because our novel morphology-based gene trap method is generally applicable, this method is promising for uncovering novel genes involved in the function of interest in any cell lines.—Hashimoto, Y., Muramatsu, K., Kunii, M., Yoshimura, S., Yamada, M., Sato, T., Ishida, Y., Harada, R., Harada, A. Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector.
publisher	Federation of American Societies for Experimental Biology
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475256/
_version_	1611917274779746304

Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector

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