Use of Biotinylated Ubiquitin for Analysis of Rat Brain Mitochondrial Proteome and Interactome

Applicability of in vitro biotinylated ubiquitin for evaluation of endogenous ubiquitin conjugation and analysis of ubiquitin-associated protein-protein interactions has been investigated. Incubation of rat brain mitochondria with biotinylated ubiquitin followed by affinity chromatography on avidin-...

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Bibliographic Details
Main Authors: Buneeva, Olga A., Medvedeva, Marina V., Kopylov, Arthur T., Zgoda, Victor G., Medvedev, Alexei E.
Format: Online
Language:English
Published: Molecular Diversity Preservation International (MDPI) 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472765/