Nestin- and Doublecortin-Positive Cells Reside in Adult Spinal Cord Meninges and Participate in Injury-Induced Parenchymal Reaction

Adult spinal cord has little regenerative potential, thus limiting patient recovery following injury. In this study, we describe a new population of cells resident in the adult rat spinal cord meninges that express the neural stem/precursor markers nestin and doublecortin. Furthermore, from dissocia...

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Main Authors:	Decimo, Ilaria, Bifari, Francesco, Rodriguez, Francisco Javier, Malpeli, Giorgio, Dolci, Sissi, Lavarini, Valentina, Pretto, Silvia, Vasquez, Sandra, Sciancalepore, Marina, Montalbano, Alberto, Berton, Valeria, Krampera, Mauro, Fumagalli, Guido
Format:	Online
Language:	English
Published:	Wiley Subscription Services, Inc., A Wiley Company 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3468739/

id	pubmed-3468739
recordtype	oai_dc
spelling	pubmed-34687392012-10-17 Nestin- and Doublecortin-Positive Cells Reside in Adult Spinal Cord Meninges and Participate in Injury-Induced Parenchymal Reaction Decimo, Ilaria Bifari, Francesco Rodriguez, Francisco Javier Malpeli, Giorgio Dolci, Sissi Lavarini, Valentina Pretto, Silvia Vasquez, Sandra Sciancalepore, Marina Montalbano, Alberto Berton, Valeria Krampera, Mauro Fumagalli, Guido Tissue-Specific Stem Cells Adult spinal cord has little regenerative potential, thus limiting patient recovery following injury. In this study, we describe a new population of cells resident in the adult rat spinal cord meninges that express the neural stem/precursor markers nestin and doublecortin. Furthermore, from dissociated meningeal tissue a neural stem cell population was cultured in vitro and subsequently shown to differentiate into functional neurons or mature oligodendrocytes. Proliferation rate and number of nestin- and doublecortin-positive cells increased in vivo in meninges following spinal cord injury. By using a lentivirus-labeling approach, we show that meningeal cells, including nestin- and doublecortin-positive cells, migrate in the spinal cord parenchyma and contribute to the glial scar formation. Our data emphasize the multiple roles of meninges in the reaction of the parenchyma to trauma and indicate for the first time that spinal cord meninges are potential niches harboring stem/precursor cells that can be activated by injury. Meninges may be considered as a new source of adult stem/precursor cells to be further tested for use in regenerative medicine applied to neurological disorders, including repair from spinal cord injury. Stem Cells 2011;29:2062–2076. Wiley Subscription Services, Inc., A Wiley Company 2011-12 2011-10-28 /pmc/articles/PMC3468739/ /pubmed/22038821 http://dx.doi.org/10.1002/stem.766 Text en Copyright © 2011 AlphaMed Press http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Decimo, Ilaria Bifari, Francesco Rodriguez, Francisco Javier Malpeli, Giorgio Dolci, Sissi Lavarini, Valentina Pretto, Silvia Vasquez, Sandra Sciancalepore, Marina Montalbano, Alberto Berton, Valeria Krampera, Mauro Fumagalli, Guido
spellingShingle	Decimo, Ilaria Bifari, Francesco Rodriguez, Francisco Javier Malpeli, Giorgio Dolci, Sissi Lavarini, Valentina Pretto, Silvia Vasquez, Sandra Sciancalepore, Marina Montalbano, Alberto Berton, Valeria Krampera, Mauro Fumagalli, Guido Nestin- and Doublecortin-Positive Cells Reside in Adult Spinal Cord Meninges and Participate in Injury-Induced Parenchymal Reaction
author_facet	Decimo, Ilaria Bifari, Francesco Rodriguez, Francisco Javier Malpeli, Giorgio Dolci, Sissi Lavarini, Valentina Pretto, Silvia Vasquez, Sandra Sciancalepore, Marina Montalbano, Alberto Berton, Valeria Krampera, Mauro Fumagalli, Guido
author_sort	Decimo, Ilaria
title	Nestin- and Doublecortin-Positive Cells Reside in Adult Spinal Cord Meninges and Participate in Injury-Induced Parenchymal Reaction
title_short	Nestin- and Doublecortin-Positive Cells Reside in Adult Spinal Cord Meninges and Participate in Injury-Induced Parenchymal Reaction
title_full	Nestin- and Doublecortin-Positive Cells Reside in Adult Spinal Cord Meninges and Participate in Injury-Induced Parenchymal Reaction
title_fullStr	Nestin- and Doublecortin-Positive Cells Reside in Adult Spinal Cord Meninges and Participate in Injury-Induced Parenchymal Reaction
title_full_unstemmed	Nestin- and Doublecortin-Positive Cells Reside in Adult Spinal Cord Meninges and Participate in Injury-Induced Parenchymal Reaction
title_sort	nestin- and doublecortin-positive cells reside in adult spinal cord meninges and participate in injury-induced parenchymal reaction
description	Adult spinal cord has little regenerative potential, thus limiting patient recovery following injury. In this study, we describe a new population of cells resident in the adult rat spinal cord meninges that express the neural stem/precursor markers nestin and doublecortin. Furthermore, from dissociated meningeal tissue a neural stem cell population was cultured in vitro and subsequently shown to differentiate into functional neurons or mature oligodendrocytes. Proliferation rate and number of nestin- and doublecortin-positive cells increased in vivo in meninges following spinal cord injury. By using a lentivirus-labeling approach, we show that meningeal cells, including nestin- and doublecortin-positive cells, migrate in the spinal cord parenchyma and contribute to the glial scar formation. Our data emphasize the multiple roles of meninges in the reaction of the parenchyma to trauma and indicate for the first time that spinal cord meninges are potential niches harboring stem/precursor cells that can be activated by injury. Meninges may be considered as a new source of adult stem/precursor cells to be further tested for use in regenerative medicine applied to neurological disorders, including repair from spinal cord injury. Stem Cells 2011;29:2062–2076.
publisher	Wiley Subscription Services, Inc., A Wiley Company
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3468739/
_version_	1611915226197786624

Nestin- and Doublecortin-Positive Cells Reside in Adult Spinal Cord Meninges and Participate in Injury-Induced Parenchymal Reaction

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