Vitamin A Deficiency Induces Congenital Spinal Deformities in Rats

Most cases of congenital spinal deformities were sporadic and without strong evidence of heritability. The etiology of congenital spinal deformities is still elusive and assumed to be multi-factorial. The current study seeks to elucidate the effect of maternal vitamin A deficiency and the production...

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Main Authors:	Li, Zheng, Shen, Jianxiong, Wu, William Ka Kei, Wang, Xiaojuan, Liang, Jinqian, Qiu, Guixing, Liu, Jiaming
Format:	Online
Language:	English
Published:	Public Library of Science 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465343/

id	pubmed-3465343
recordtype	oai_dc
spelling	pubmed-34653432012-10-15 Vitamin A Deficiency Induces Congenital Spinal Deformities in Rats Li, Zheng Shen, Jianxiong Wu, William Ka Kei Wang, Xiaojuan Liang, Jinqian Qiu, Guixing Liu, Jiaming Research Article Most cases of congenital spinal deformities were sporadic and without strong evidence of heritability. The etiology of congenital spinal deformities is still elusive and assumed to be multi-factorial. The current study seeks to elucidate the effect of maternal vitamin A deficiency and the production of congenital spinal deformities in the offsping. Thirty two female rats were randomized into two groups: control group, which was fed a normal diet; vitamin A deficient group, which were given vitamin A-deficient diet from at least 2 weeks before mating till delivery. Three random neonatal rats from each group were killed the next day of parturition. Female rats were fed an AIN-93G diet sufficient in vitamin A to feed the rest of neonates for two weeks until euthanasia. Serum levels of vitamin A were assessed in the adult and filial rats. Anteroposterior (AP) spine radiographs were obtained at week 2 after delivery to evaluate the presence of the skeletal abnormalities especially of spinal deformities. Liver and vertebral body expression of retinaldehyde dehydrogenase (RALDHs) and RARs mRNA was assessed by reverse transcription-real time PCR. VAD neonates displayed many skeletal malformations in the cervical, thoracic, the pelvic and sacral and limbs regions. The incidence of congenital scoliosis was 13.79% (8/58) in the filial rats of vitamin A deficiency group and 0% in the control group. Furthermore, vitamin A deficiency negatively regulate the liver and verterbral body mRNA levels of RALDH1, RALDH2, RALDH3, RAR-α, RAR-β and RAR-γ. Vitamin A deficiency in pregnancy may induce congenital spinal deformities in the postnatal rats. The decreases of RALDHs and RARs mRNA expression induced by vitamin A deprivation suggest that vertebral birth defects may be caused by a defect in RA signaling pathway during somitogenesis. Public Library of Science 2012-10-05 /pmc/articles/PMC3465343/ /pubmed/23071590 http://dx.doi.org/10.1371/journal.pone.0046565 Text en © 2012 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Li, Zheng Shen, Jianxiong Wu, William Ka Kei Wang, Xiaojuan Liang, Jinqian Qiu, Guixing Liu, Jiaming
spellingShingle	Li, Zheng Shen, Jianxiong Wu, William Ka Kei Wang, Xiaojuan Liang, Jinqian Qiu, Guixing Liu, Jiaming Vitamin A Deficiency Induces Congenital Spinal Deformities in Rats
author_facet	Li, Zheng Shen, Jianxiong Wu, William Ka Kei Wang, Xiaojuan Liang, Jinqian Qiu, Guixing Liu, Jiaming
author_sort	Li, Zheng
title	Vitamin A Deficiency Induces Congenital Spinal Deformities in Rats
title_short	Vitamin A Deficiency Induces Congenital Spinal Deformities in Rats
title_full	Vitamin A Deficiency Induces Congenital Spinal Deformities in Rats
title_fullStr	Vitamin A Deficiency Induces Congenital Spinal Deformities in Rats
title_full_unstemmed	Vitamin A Deficiency Induces Congenital Spinal Deformities in Rats
title_sort	vitamin a deficiency induces congenital spinal deformities in rats
description	Most cases of congenital spinal deformities were sporadic and without strong evidence of heritability. The etiology of congenital spinal deformities is still elusive and assumed to be multi-factorial. The current study seeks to elucidate the effect of maternal vitamin A deficiency and the production of congenital spinal deformities in the offsping. Thirty two female rats were randomized into two groups: control group, which was fed a normal diet; vitamin A deficient group, which were given vitamin A-deficient diet from at least 2 weeks before mating till delivery. Three random neonatal rats from each group were killed the next day of parturition. Female rats were fed an AIN-93G diet sufficient in vitamin A to feed the rest of neonates for two weeks until euthanasia. Serum levels of vitamin A were assessed in the adult and filial rats. Anteroposterior (AP) spine radiographs were obtained at week 2 after delivery to evaluate the presence of the skeletal abnormalities especially of spinal deformities. Liver and vertebral body expression of retinaldehyde dehydrogenase (RALDHs) and RARs mRNA was assessed by reverse transcription-real time PCR. VAD neonates displayed many skeletal malformations in the cervical, thoracic, the pelvic and sacral and limbs regions. The incidence of congenital scoliosis was 13.79% (8/58) in the filial rats of vitamin A deficiency group and 0% in the control group. Furthermore, vitamin A deficiency negatively regulate the liver and verterbral body mRNA levels of RALDH1, RALDH2, RALDH3, RAR-α, RAR-β and RAR-γ. Vitamin A deficiency in pregnancy may induce congenital spinal deformities in the postnatal rats. The decreases of RALDHs and RARs mRNA expression induced by vitamin A deprivation suggest that vertebral birth defects may be caused by a defect in RA signaling pathway during somitogenesis.
publisher	Public Library of Science
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465343/
_version_	1611914153775071232

Vitamin A Deficiency Induces Congenital Spinal Deformities in Rats

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