The Transcriptome Profile of the Mosquito Culex quinquefasciatus following Permethrin Selection

To gain valuable insights into the gene interaction and the complex regulation system involved in the development of insecticide resistance in mosquitoes Culex quinquefasciatus, we conducted a whole transcriptome analysis of Culex mosquitoes following permethrin selection. Gene expression profiles f...

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Main Authors:	Reid, William R., Zhang, Lee, Liu, Feng, Liu, Nannan
Format:	Online
Language:	English
Published:	Public Library of Science 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465273/

id	pubmed-3465273
recordtype	oai_dc
spelling	pubmed-34652732012-10-15 The Transcriptome Profile of the Mosquito Culex quinquefasciatus following Permethrin Selection Reid, William R. Zhang, Lee Liu, Feng Liu, Nannan Research Article To gain valuable insights into the gene interaction and the complex regulation system involved in the development of insecticide resistance in mosquitoes Culex quinquefasciatus, we conducted a whole transcriptome analysis of Culex mosquitoes following permethrin selection. Gene expression profiles for the lower resistant parental mosquito strain HAmCqG0 and their permethrin-selected high resistant offspring HAmCqG8 were compared and a total of 367 and 3982 genes were found to be up- and down-regulated, respectively, in HAmCqG8, indicating that multiple genes are involved in response to permethrin selection. However, a similar overall cumulative gene expression abundance was identified between up- and down-regulated genes in HAmCqG8 mosquitoes following permethrin selection, suggesting a homeostatic response to insecticides through a balancing of the up- and down-regulation of the genes. While structural and/or cuticular structural functions were the only two enriched GO terms for down-regulated genes, the enriched GO terms obtained for the up-regulated genes occurred primarily among the catalytic and metabolic functions where they represented three functional categories: electron carrier activity, binding, and catalytic activity. Interestingly, the functional GO terms in these three functional categories were overwhelmingly overrepresented in P450s and proteases/serine proteases. The important role played by P450s in the development of insecticide resistance has been extensively studied but the function of proteases/serine proteases in resistance is less well understood. Hence, the characterization of the functions of these proteins, including their digestive, catalytic and proteinase activities; regulation of signaling transduction and protein trafficking, immunity and storage; and their precise function in the development of insecticide resistance in mosquitoes will provide new insights into how genes are interconnected and regulated in resistance. Public Library of Science 2012-10-05 /pmc/articles/PMC3465273/ /pubmed/23071746 http://dx.doi.org/10.1371/journal.pone.0047163 Text en © 2012 Reid et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Reid, William R. Zhang, Lee Liu, Feng Liu, Nannan
spellingShingle	Reid, William R. Zhang, Lee Liu, Feng Liu, Nannan The Transcriptome Profile of the Mosquito Culex quinquefasciatus following Permethrin Selection
author_facet	Reid, William R. Zhang, Lee Liu, Feng Liu, Nannan
author_sort	Reid, William R.
title	The Transcriptome Profile of the Mosquito Culex quinquefasciatus following Permethrin Selection
title_short	The Transcriptome Profile of the Mosquito Culex quinquefasciatus following Permethrin Selection
title_full	The Transcriptome Profile of the Mosquito Culex quinquefasciatus following Permethrin Selection
title_fullStr	The Transcriptome Profile of the Mosquito Culex quinquefasciatus following Permethrin Selection
title_full_unstemmed	The Transcriptome Profile of the Mosquito Culex quinquefasciatus following Permethrin Selection
title_sort	transcriptome profile of the mosquito culex quinquefasciatus following permethrin selection
description	To gain valuable insights into the gene interaction and the complex regulation system involved in the development of insecticide resistance in mosquitoes Culex quinquefasciatus, we conducted a whole transcriptome analysis of Culex mosquitoes following permethrin selection. Gene expression profiles for the lower resistant parental mosquito strain HAmCqG0 and their permethrin-selected high resistant offspring HAmCqG8 were compared and a total of 367 and 3982 genes were found to be up- and down-regulated, respectively, in HAmCqG8, indicating that multiple genes are involved in response to permethrin selection. However, a similar overall cumulative gene expression abundance was identified between up- and down-regulated genes in HAmCqG8 mosquitoes following permethrin selection, suggesting a homeostatic response to insecticides through a balancing of the up- and down-regulation of the genes. While structural and/or cuticular structural functions were the only two enriched GO terms for down-regulated genes, the enriched GO terms obtained for the up-regulated genes occurred primarily among the catalytic and metabolic functions where they represented three functional categories: electron carrier activity, binding, and catalytic activity. Interestingly, the functional GO terms in these three functional categories were overwhelmingly overrepresented in P450s and proteases/serine proteases. The important role played by P450s in the development of insecticide resistance has been extensively studied but the function of proteases/serine proteases in resistance is less well understood. Hence, the characterization of the functions of these proteins, including their digestive, catalytic and proteinase activities; regulation of signaling transduction and protein trafficking, immunity and storage; and their precise function in the development of insecticide resistance in mosquitoes will provide new insights into how genes are interconnected and regulated in resistance.
publisher	Public Library of Science
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465273/
_version_	1611914111930597376

The Transcriptome Profile of the Mosquito Culex quinquefasciatus following Permethrin Selection

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