Rif2 Promotes a Telomere Fold-Back Structure through Rpd3L Recruitment in Budding Yeast

Using a genome-wide screening approach, we have established the genetic requirements for proper telomere structure in Saccharomyces cerevisiae. We uncovered 112 genes, many of which have not previously been implicated in telomere function, that are required to form a fold-back structure at chromosom...

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Main Authors:	Poschke, Heiko, Dees, Martina, Chang, Michael, Amberkar, Sandeep, Kaderali, Lars, Rothstein, Rodney, Luke, Brian
Format:	Online
Language:	English
Published:	Public Library of Science 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447961/

id	pubmed-3447961
recordtype	oai_dc
spelling	pubmed-34479612012-10-01 Rif2 Promotes a Telomere Fold-Back Structure through Rpd3L Recruitment in Budding Yeast Poschke, Heiko Dees, Martina Chang, Michael Amberkar, Sandeep Kaderali, Lars Rothstein, Rodney Luke, Brian Research Article Using a genome-wide screening approach, we have established the genetic requirements for proper telomere structure in Saccharomyces cerevisiae. We uncovered 112 genes, many of which have not previously been implicated in telomere function, that are required to form a fold-back structure at chromosome ends. Among other biological processes, lysine deacetylation, through the Rpd3L, Rpd3S, and Hda1 complexes, emerged as being a critical regulator of telomere structure. The telomeric-bound protein, Rif2, was also found to promote a telomere fold-back through the recruitment of Rpd3L to telomeres. In the absence of Rpd3 function, telomeres have an increased susceptibility to nucleolytic degradation, telomere loss, and the initiation of premature senescence, suggesting that an Rpd3-mediated structure may have protective functions. Together these data reveal that multiple genetic pathways may directly or indirectly impinge on telomere structure, thus broadening the potential targets available to manipulate telomere function. Public Library of Science 2012-09-20 /pmc/articles/PMC3447961/ /pubmed/23028367 http://dx.doi.org/10.1371/journal.pgen.1002960 Text en © 2012 Poschke et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Poschke, Heiko Dees, Martina Chang, Michael Amberkar, Sandeep Kaderali, Lars Rothstein, Rodney Luke, Brian
spellingShingle	Poschke, Heiko Dees, Martina Chang, Michael Amberkar, Sandeep Kaderali, Lars Rothstein, Rodney Luke, Brian Rif2 Promotes a Telomere Fold-Back Structure through Rpd3L Recruitment in Budding Yeast
author_facet	Poschke, Heiko Dees, Martina Chang, Michael Amberkar, Sandeep Kaderali, Lars Rothstein, Rodney Luke, Brian
author_sort	Poschke, Heiko
title	Rif2 Promotes a Telomere Fold-Back Structure through Rpd3L Recruitment in Budding Yeast
title_short	Rif2 Promotes a Telomere Fold-Back Structure through Rpd3L Recruitment in Budding Yeast
title_full	Rif2 Promotes a Telomere Fold-Back Structure through Rpd3L Recruitment in Budding Yeast
title_fullStr	Rif2 Promotes a Telomere Fold-Back Structure through Rpd3L Recruitment in Budding Yeast
title_full_unstemmed	Rif2 Promotes a Telomere Fold-Back Structure through Rpd3L Recruitment in Budding Yeast
title_sort	rif2 promotes a telomere fold-back structure through rpd3l recruitment in budding yeast
description	Using a genome-wide screening approach, we have established the genetic requirements for proper telomere structure in Saccharomyces cerevisiae. We uncovered 112 genes, many of which have not previously been implicated in telomere function, that are required to form a fold-back structure at chromosome ends. Among other biological processes, lysine deacetylation, through the Rpd3L, Rpd3S, and Hda1 complexes, emerged as being a critical regulator of telomere structure. The telomeric-bound protein, Rif2, was also found to promote a telomere fold-back through the recruitment of Rpd3L to telomeres. In the absence of Rpd3 function, telomeres have an increased susceptibility to nucleolytic degradation, telomere loss, and the initiation of premature senescence, suggesting that an Rpd3-mediated structure may have protective functions. Together these data reveal that multiple genetic pathways may directly or indirectly impinge on telomere structure, thus broadening the potential targets available to manipulate telomere function.
publisher	Public Library of Science
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447961/
_version_	1611910758508003328

Rif2 Promotes a Telomere Fold-Back Structure through Rpd3L Recruitment in Budding Yeast

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