Imaging granularity of leukocytes with third harmonic generation microscopy

Using third harmonic generation (THG) microscopy, we demonstrate that granularity differences of leukocytes can be revealed without a label. Excited by a 1230 nm femtosecond laser, THG signals were generated at a significantly higher level in neutrophils than other mononuclear cells, whereas signals...

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Bibliographic Details
Main Authors: Tsai, Cheng-Kun, Chen, Yu-Shing, Wu, Pei-Chun, Hsieh, Tsung-Yuan, Liu, Han-Wen, Yeh, Chiou-Yueh, Lin, Win-Li, Chia, Jean-San, Liu, Tzu-Ming
Format: Online
Language:English
Published: Optical Society of America 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447564/