Efficient and robust RNA-seq process for cultured bacteria and complex community transcriptomes
We have developed a process for transcriptome analysis of bacterial communities that accommodates both intact and fragmented starting RNA and combines efficient rRNA removal with strand-specific RNA-seq. We applied this approach to an RNA mixture derived from three diverse cultured bacterial species...
Main Authors: | , , , , , , , , , , , , |
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Format: | Online |
Language: | English |
Published: |
BioMed Central
2012
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3439974/ |