Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy

We recently found that chemokine-driven peritoneal cell aggregation is the primary mechanism of postoperative adhesion in a mouse model. To investigate this in humans, paired samples of peritoneal lavage fluid were obtained from seven patients immediately after incision (preoperative) and before clo...

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Main Authors:	Kawashima, Rei, Kawamura, Yuki I., Oshio, Tomoyuki, Mizutani, Noriko, Okada, Toshihiko, Kawamura, Yutaka J., Konishi, Fumio, Dohi, Taeko
Format:	Online
Language:	English
Published:	Taylor & Francis 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431549/

id	pubmed-3431549
recordtype	oai_dc
spelling	pubmed-34315492012-08-31 Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy Kawashima, Rei Kawamura, Yuki I. Oshio, Tomoyuki Mizutani, Noriko Okada, Toshihiko Kawamura, Yutaka J. Konishi, Fumio Dohi, Taeko Research Article We recently found that chemokine-driven peritoneal cell aggregation is the primary mechanism of postoperative adhesion in a mouse model. To investigate this in humans, paired samples of peritoneal lavage fluid were obtained from seven patients immediately after incision (preoperative) and before closure (postoperative), and were assayed for the presence of 27 cytokines and chemokines using multiplex beads assay. As a result, IL-6 and CCL5 showed the most striking increase during operation. Recombinant CCL5 or lavage fluid induced chemotaxis of human peripheral blood mononuclear cells. We propose that CCL5 is possibly involved in the mechanism of postoperative adhesion in humans. Taylor & Francis 2012-06-27 2012-07 /pmc/articles/PMC3431549/ /pubmed/22738652 http://dx.doi.org/10.1080/15321819.2011.638409 Text en Copyright © Taylor & Francis Group, LLC http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf This is an open access article distributed under the Supplemental Terms and Conditions for iOpenAccess articles published in Taylor & Francis journals (http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Kawashima, Rei Kawamura, Yuki I. Oshio, Tomoyuki Mizutani, Noriko Okada, Toshihiko Kawamura, Yutaka J. Konishi, Fumio Dohi, Taeko
spellingShingle	Kawashima, Rei Kawamura, Yuki I. Oshio, Tomoyuki Mizutani, Noriko Okada, Toshihiko Kawamura, Yutaka J. Konishi, Fumio Dohi, Taeko Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy
author_facet	Kawashima, Rei Kawamura, Yuki I. Oshio, Tomoyuki Mizutani, Noriko Okada, Toshihiko Kawamura, Yutaka J. Konishi, Fumio Dohi, Taeko
author_sort	Kawashima, Rei
title	Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy
title_short	Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy
title_full	Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy
title_fullStr	Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy
title_full_unstemmed	Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy
title_sort	comprehensive analysis of chemokines and cytokines secreted in the peritoneal cavity during laparotomy
description	We recently found that chemokine-driven peritoneal cell aggregation is the primary mechanism of postoperative adhesion in a mouse model. To investigate this in humans, paired samples of peritoneal lavage fluid were obtained from seven patients immediately after incision (preoperative) and before closure (postoperative), and were assayed for the presence of 27 cytokines and chemokines using multiplex beads assay. As a result, IL-6 and CCL5 showed the most striking increase during operation. Recombinant CCL5 or lavage fluid induced chemotaxis of human peripheral blood mononuclear cells. We propose that CCL5 is possibly involved in the mechanism of postoperative adhesion in humans.
publisher	Taylor & Francis
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431549/
_version_	1611553489578622976

Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy

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