Stratified active archaeal communities in the sediments of Jiulong River estuary, China

Here the composition of total and active archaeal communities in a sediment core of Jiulong River estuary at Fujian Province, Southern China was reported. Profiles of CH4 and SO2−4 concentrations from the sediment core indicated the existence of a sulfate-methane transition zone (SMTZ) in which sulf...

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Main Authors:	Li, Qianqian, Wang, Fengping, Chen, Zhiwei, Yin, Xijie, Xiao, Xiang
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430981/

id	pubmed-3430981
recordtype	oai_dc
spelling	pubmed-34309812012-09-11 Stratified active archaeal communities in the sediments of Jiulong River estuary, China Li, Qianqian Wang, Fengping Chen, Zhiwei Yin, Xijie Xiao, Xiang Microbiology Here the composition of total and active archaeal communities in a sediment core of Jiulong River estuary at Fujian Province, Southern China was reported. Profiles of CH4 and SO2−4 concentrations from the sediment core indicated the existence of a sulfate-methane transition zone (SMTZ) in which sulfate reduction-coupled anaerobic oxidation of methane (AOM) occurs. Accordingly, three sediment layers (16–18.5 cm, 71–73.5 cm, and 161–163.5 cm) from the 1.2 m sediment core were sectioned and named top, middle and bottom, respectively. Total DNA and RNA of each layer were extracted and used for clone libraries and sequence analysis of 16S rRNA genes, the reverse transcription (RT)-PCR products of 16S rRNA and methyl CoM reductase alpha subunit (mcrA) genes. Phylogenetic analysis indicated that archaeal communities of the three layers were dominated by the Miscellaneous Crenarchaeotal Group (MCG) whose ecological functions were still unknown. The MCG could be further divided into seven subgroups, named MCG-A, B, C, D, E, F, and G. MCG-A and MCG-G were the most active groups in the estuarine sediments. Known anaerobic methanotrophic archaea (ANMEs) were only found as minor components in these estuarine archaeal communities. This study, together with the studies of deep subsurface sediments, would be a very good start point to target and compare the specific active archaeal groups and their roles in the dark, deep subsurface sediment environments. Frontiers Media S.A. 2012-08-30 /pmc/articles/PMC3430981/ /pubmed/22969752 http://dx.doi.org/10.3389/fmicb.2012.00311 Text en Copyright © 2012 Li, Wang, Chen, Yin and Xiao. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Li, Qianqian Wang, Fengping Chen, Zhiwei Yin, Xijie Xiao, Xiang
spellingShingle	Li, Qianqian Wang, Fengping Chen, Zhiwei Yin, Xijie Xiao, Xiang Stratified active archaeal communities in the sediments of Jiulong River estuary, China
author_facet	Li, Qianqian Wang, Fengping Chen, Zhiwei Yin, Xijie Xiao, Xiang
author_sort	Li, Qianqian
title	Stratified active archaeal communities in the sediments of Jiulong River estuary, China
title_short	Stratified active archaeal communities in the sediments of Jiulong River estuary, China
title_full	Stratified active archaeal communities in the sediments of Jiulong River estuary, China
title_fullStr	Stratified active archaeal communities in the sediments of Jiulong River estuary, China
title_full_unstemmed	Stratified active archaeal communities in the sediments of Jiulong River estuary, China
title_sort	stratified active archaeal communities in the sediments of jiulong river estuary, china
description	Here the composition of total and active archaeal communities in a sediment core of Jiulong River estuary at Fujian Province, Southern China was reported. Profiles of CH4 and SO2−4 concentrations from the sediment core indicated the existence of a sulfate-methane transition zone (SMTZ) in which sulfate reduction-coupled anaerobic oxidation of methane (AOM) occurs. Accordingly, three sediment layers (16–18.5 cm, 71–73.5 cm, and 161–163.5 cm) from the 1.2 m sediment core were sectioned and named top, middle and bottom, respectively. Total DNA and RNA of each layer were extracted and used for clone libraries and sequence analysis of 16S rRNA genes, the reverse transcription (RT)-PCR products of 16S rRNA and methyl CoM reductase alpha subunit (mcrA) genes. Phylogenetic analysis indicated that archaeal communities of the three layers were dominated by the Miscellaneous Crenarchaeotal Group (MCG) whose ecological functions were still unknown. The MCG could be further divided into seven subgroups, named MCG-A, B, C, D, E, F, and G. MCG-A and MCG-G were the most active groups in the estuarine sediments. Known anaerobic methanotrophic archaea (ANMEs) were only found as minor components in these estuarine archaeal communities. This study, together with the studies of deep subsurface sediments, would be a very good start point to target and compare the specific active archaeal groups and their roles in the dark, deep subsurface sediment environments.
publisher	Frontiers Media S.A.
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430981/
_version_	1611553275241299968

Stratified active archaeal communities in the sediments of Jiulong River estuary, China

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