Genetic Diversity of the Edible Mushroom Pleurotus sp. by Amplified Fragment Length Polymorphism

Pleurotus strains are the most important fungi used in the agricultural industry. The exact characterization and identification of Pleurotus species is fundamental for correct identification of the individuals and exploiting their full potential in food industry. The amplified fragment length polymo...

Full description

Bibliographic Details
Main Authors:	Pawlik, Anna, Janusz, Grzegorz, Koszerny, Joanna, Małek, Wanda, Rogalski, Jerzy
Format:	Online
Language:	English
Published:	Springer-Verlag 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426667/

id	pubmed-3426667
recordtype	oai_dc
spelling	pubmed-34266672012-08-29 Genetic Diversity of the Edible Mushroom Pleurotus sp. by Amplified Fragment Length Polymorphism Pawlik, Anna Janusz, Grzegorz Koszerny, Joanna Małek, Wanda Rogalski, Jerzy Article Pleurotus strains are the most important fungi used in the agricultural industry. The exact characterization and identification of Pleurotus species is fundamental for correct identification of the individuals and exploiting their full potential in food industry. The amplified fragment length polymorphism (AFLP) method was applied for genomic fingerprinting of 21 Pleurotus isolates of Asian and European origin. Using one PstI restriction endonuclease and four selective primers in an AFLP assay, 371 DNA fragments were generated, including 308 polymorphic bands. The AFLP profiles were found to be highly specific for each strain and they unambiguously distinguished 21 Pleurotus sp. fungi. The coefficient of Jaccard’s genome profile similarity between the analyzed strains ranged from 0.0 (Pleurotus sp. I vs. P. sajor-caju 237 and P. eryngii 238) to 0.750 (P. ostreatus 246 vs. P. ostreatus 248), and the average was 0.378. The AFLP-based dendrogram generated by the UPGMA method grouped all the Pleurotus fungi studied into two major clusters and one independent lineage located on the outskirt of the tree occupied by naturally growing Pleurotus species strain I. The results of the present study suggest the possible applicability of the AFLP-PstI method in effective identification and molecular characterization of Pleurotus sp. strains. Springer-Verlag 2012-07-06 2012-10 /pmc/articles/PMC3426667/ /pubmed/22767319 http://dx.doi.org/10.1007/s00284-012-0175-7 Text en © The Author(s) 2012
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Pawlik, Anna Janusz, Grzegorz Koszerny, Joanna Małek, Wanda Rogalski, Jerzy
spellingShingle	Pawlik, Anna Janusz, Grzegorz Koszerny, Joanna Małek, Wanda Rogalski, Jerzy Genetic Diversity of the Edible Mushroom Pleurotus sp. by Amplified Fragment Length Polymorphism
author_facet	Pawlik, Anna Janusz, Grzegorz Koszerny, Joanna Małek, Wanda Rogalski, Jerzy
author_sort	Pawlik, Anna
title	Genetic Diversity of the Edible Mushroom Pleurotus sp. by Amplified Fragment Length Polymorphism
title_short	Genetic Diversity of the Edible Mushroom Pleurotus sp. by Amplified Fragment Length Polymorphism
title_full	Genetic Diversity of the Edible Mushroom Pleurotus sp. by Amplified Fragment Length Polymorphism
title_fullStr	Genetic Diversity of the Edible Mushroom Pleurotus sp. by Amplified Fragment Length Polymorphism
title_full_unstemmed	Genetic Diversity of the Edible Mushroom Pleurotus sp. by Amplified Fragment Length Polymorphism
title_sort	genetic diversity of the edible mushroom pleurotus sp. by amplified fragment length polymorphism
description	Pleurotus strains are the most important fungi used in the agricultural industry. The exact characterization and identification of Pleurotus species is fundamental for correct identification of the individuals and exploiting their full potential in food industry. The amplified fragment length polymorphism (AFLP) method was applied for genomic fingerprinting of 21 Pleurotus isolates of Asian and European origin. Using one PstI restriction endonuclease and four selective primers in an AFLP assay, 371 DNA fragments were generated, including 308 polymorphic bands. The AFLP profiles were found to be highly specific for each strain and they unambiguously distinguished 21 Pleurotus sp. fungi. The coefficient of Jaccard’s genome profile similarity between the analyzed strains ranged from 0.0 (Pleurotus sp. I vs. P. sajor-caju 237 and P. eryngii 238) to 0.750 (P. ostreatus 246 vs. P. ostreatus 248), and the average was 0.378. The AFLP-based dendrogram generated by the UPGMA method grouped all the Pleurotus fungi studied into two major clusters and one independent lineage located on the outskirt of the tree occupied by naturally growing Pleurotus species strain I. The results of the present study suggest the possible applicability of the AFLP-PstI method in effective identification and molecular characterization of Pleurotus sp. strains.
publisher	Springer-Verlag
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426667/
_version_	1611551962612891648

Genetic Diversity of the Edible Mushroom Pleurotus sp. by Amplified Fragment Length Polymorphism

Similar Items