Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection

The flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-term strategy to co...

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Main Authors:	Martins, Vicente P., Pinheiro, Carina S., Figueiredo, Barbara C. P., Assis, Natan R. G., Morais, Suellen B., Caliari, Marcelo V., Azevedo, Vasco, Castro-Borges, William, Wilson, R. Alan, Oliveira, Sergio C.
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426240/

id	pubmed-3426240
recordtype	oai_dc
spelling	pubmed-34262402012-08-27 Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection Martins, Vicente P. Pinheiro, Carina S. Figueiredo, Barbara C. P. Assis, Natan R. G. Morais, Suellen B. Caliari, Marcelo V. Azevedo, Vasco Castro-Borges, William Wilson, R. Alan Oliveira, Sergio C. Research Article The flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-term strategy to control schistosomiasis is through immunization with an antischistosomiasis vaccine combined with drug treatment. In the search for potential vaccine candidates, numerous tegument antigens have been assessed. As the major interface between parasite and mammalian host, the tegument plays crucial roles in the establishment and further course of schistosomiasis. Herein, we evaluated the potential of a GPI fraction, containing representative molecules located on the outer surface of adult worms, as vaccine candidate. Immunization of mice with GPI-anchored proteins induced a mixed Th1/Th2 type of immune response with production of IFN-γ and TNF-α, and low levels of IL-5 into the supernatant of splenocyte cultures. The protection engendered by this vaccination protocol was confirmed by 42% reduction in worm burden, 45% reduction in eggs per gram of hepatic tissue, 29% reduction in the number of granulomas per area, and 53% reduction in the granuloma fibrosis. Taken together, the data herein support the potential of surface-exposed GPI-anchored antigens from the S. mansoni tegument as vaccine candidate. Hindawi Publishing Corporation 2012 2012-08-15 /pmc/articles/PMC3426240/ /pubmed/22927873 http://dx.doi.org/10.1155/2012/962538 Text en Copyright © 2012 Vicente P. Martins et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Martins, Vicente P. Pinheiro, Carina S. Figueiredo, Barbara C. P. Assis, Natan R. G. Morais, Suellen B. Caliari, Marcelo V. Azevedo, Vasco Castro-Borges, William Wilson, R. Alan Oliveira, Sergio C.
spellingShingle	Martins, Vicente P. Pinheiro, Carina S. Figueiredo, Barbara C. P. Assis, Natan R. G. Morais, Suellen B. Caliari, Marcelo V. Azevedo, Vasco Castro-Borges, William Wilson, R. Alan Oliveira, Sergio C. Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection
author_facet	Martins, Vicente P. Pinheiro, Carina S. Figueiredo, Barbara C. P. Assis, Natan R. G. Morais, Suellen B. Caliari, Marcelo V. Azevedo, Vasco Castro-Borges, William Wilson, R. Alan Oliveira, Sergio C.
author_sort	Martins, Vicente P.
title	Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection
title_short	Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection
title_full	Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection
title_fullStr	Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection
title_full_unstemmed	Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection
title_sort	vaccination with enzymatically cleaved gpi-anchored proteins from schistosoma mansoni induces protection against challenge infection
description	The flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-term strategy to control schistosomiasis is through immunization with an antischistosomiasis vaccine combined with drug treatment. In the search for potential vaccine candidates, numerous tegument antigens have been assessed. As the major interface between parasite and mammalian host, the tegument plays crucial roles in the establishment and further course of schistosomiasis. Herein, we evaluated the potential of a GPI fraction, containing representative molecules located on the outer surface of adult worms, as vaccine candidate. Immunization of mice with GPI-anchored proteins induced a mixed Th1/Th2 type of immune response with production of IFN-γ and TNF-α, and low levels of IL-5 into the supernatant of splenocyte cultures. The protection engendered by this vaccination protocol was confirmed by 42% reduction in worm burden, 45% reduction in eggs per gram of hepatic tissue, 29% reduction in the number of granulomas per area, and 53% reduction in the granuloma fibrosis. Taken together, the data herein support the potential of surface-exposed GPI-anchored antigens from the S. mansoni tegument as vaccine candidate.
publisher	Hindawi Publishing Corporation
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426240/
_version_	1611551833439862784

Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection

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