Development and Validation of a RP-HPLC Method for Estimation of Prulifloxacin in Tablet Dosage Form
A simple, precise, rapid, accurate and economic reverse phase high performance liquid chromatographic method has been developed for the estimation of prulifloxacin in tablet dosage form. The separation was achieved by using octadecylsilane column (C18) and KH2PO4 buffer: acetonitrile adjusted to pH...
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| Format: | Online |
| Language: | English |
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Medknow Publications & Media Pvt Ltd
2011
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425072/ |
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pubmed-3425072 |
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pubmed-34250722012-08-24 Development and Validation of a RP-HPLC Method for Estimation of Prulifloxacin in Tablet Dosage Form Singh, S. Singh, U. K. Singh, R. M. Singh, G. N. Mathur, S. C. Saini, P. K. Yadav, A. Gupta, V. Duggal, D. Short Communications A simple, precise, rapid, accurate and economic reverse phase high performance liquid chromatographic method has been developed for the estimation of prulifloxacin in tablet dosage form. The separation was achieved by using octadecylsilane column (C18) and KH2PO4 buffer: acetonitrile adjusted to pH 7.3 with triethyl amine in proportion of 10:90 v/v as mobile phase, at a flow rate of 1.0 ml/min. The detection was carried out at 278 nm. The retention time of prulifloxacin was found to be 2.4 min. The limit of detection and limit of quantitation were found to be 0.14 μg/ml and 0.42 μg/ml respectively. The accuracy and reliability of the proposed method was ascertained by evaluating various validation parameters like linearity, precision, accuracy and specificity according to ICH guidelines. The proposed method provides an accurate and precise quality control tool for routine analysis of prulifloxacin in tablet dosage form. Medknow Publications & Media Pvt Ltd 2011 /pmc/articles/PMC3425072/ /pubmed/22923873 http://dx.doi.org/10.4103/0250-474X.99019 Text en Copyright: © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Singh, S. Singh, U. K. Singh, R. M. Singh, G. N. Mathur, S. C. Saini, P. K. Yadav, A. Gupta, V. Duggal, D. |
| spellingShingle |
Singh, S. Singh, U. K. Singh, R. M. Singh, G. N. Mathur, S. C. Saini, P. K. Yadav, A. Gupta, V. Duggal, D. Development and Validation of a RP-HPLC Method for Estimation of Prulifloxacin in Tablet Dosage Form |
| author_facet |
Singh, S. Singh, U. K. Singh, R. M. Singh, G. N. Mathur, S. C. Saini, P. K. Yadav, A. Gupta, V. Duggal, D. |
| author_sort |
Singh, S. |
| title |
Development and Validation of a RP-HPLC Method for Estimation of Prulifloxacin in Tablet Dosage Form |
| title_short |
Development and Validation of a RP-HPLC Method for Estimation of Prulifloxacin in Tablet Dosage Form |
| title_full |
Development and Validation of a RP-HPLC Method for Estimation of Prulifloxacin in Tablet Dosage Form |
| title_fullStr |
Development and Validation of a RP-HPLC Method for Estimation of Prulifloxacin in Tablet Dosage Form |
| title_full_unstemmed |
Development and Validation of a RP-HPLC Method for Estimation of Prulifloxacin in Tablet Dosage Form |
| title_sort |
development and validation of a rp-hplc method for estimation of prulifloxacin in tablet dosage form |
| description |
A simple, precise, rapid, accurate and economic reverse phase high performance liquid chromatographic method has been developed for the estimation of prulifloxacin in tablet dosage form. The separation was achieved by using octadecylsilane column (C18) and KH2PO4 buffer: acetonitrile adjusted to pH 7.3 with triethyl amine in proportion of 10:90 v/v as mobile phase, at a flow rate of 1.0 ml/min. The detection was carried out at 278 nm. The retention time of prulifloxacin was found to be 2.4 min. The limit of detection and limit of quantitation were found to be 0.14 μg/ml and 0.42 μg/ml respectively. The accuracy and reliability of the proposed method was ascertained by evaluating various validation parameters like linearity, precision, accuracy and specificity according to ICH guidelines. The proposed method provides an accurate and precise quality control tool for routine analysis of prulifloxacin in tablet dosage form. |
| publisher |
Medknow Publications & Media Pvt Ltd |
| publishDate |
2011 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425072/ |
| _version_ |
1611551379415891968 |