Primer3—new capabilities and interfaces

Polymerase chain reaction (PCR) is a basic molecular biology technique with a multiplicity of uses, including deoxyribonucleic acid cloning and sequencing, functional analysis of genes, diagnosis of diseases, genotyping and discovery of genetic variants. Reliable primer design is crucial for success...

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Bibliographic Details
Main Authors: Untergasser, Andreas, Cutcutache, Ioana, Koressaar, Triinu, Ye, Jian, Faircloth, Brant C., Remm, Maido, Rozen, Steven G.
Format: Online
Language:English
Published: Oxford University Press 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424584/