Targeted gene knockout by direct delivery of ZFN proteins

Zinc-finger nucleases (ZFNs) are versatile reagents that have redefined genome engineering. Realizing the full potential of this technology requires the development of safe and effective methods for delivering ZFNs into cells. We demonstrate the intrinsic cell-penetrating capabilities of the standar...

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Bibliographic Details
Main Authors: Gaj, Thomas, Guo, Jing, Kato, Yoshio, Sirk, Shannon J., Barbas, Carlos F.
Format: Online
Language:English
Published: 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424280/