Simple Methods for Generating and Detecting Locus-Specific Mutations Induced with TALENs in the Zebrafish Genome

The zebrafish is a powerful experimental system for uncovering gene function in vertebrate organisms. Nevertheless, studies in the zebrafish have been limited by the approaches available for eliminating gene function. Here we present simple and efficient methods for inducing, detecting, and recoveri...

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Bibliographic Details
Main Authors: Dahlem, Timothy J., Hoshijima, Kazuyuki, Jurynec, Michael J., Gunther, Derrick, Starker, Colby G., Locke, Alexandra S., Weis, Allison M., Voytas, Daniel F., Grunwald, David Jonah
Format: Online
Language:English
Published: Public Library of Science 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3420959/