MreA Functions in the Global Regulation of Methanogenic Pathways in Methanosarcina acetivorans

Results are presented supporting a regulatory role for the product of the MA3302 gene locus (designated MreA) previously annotated as a hypothetical protein in the methanogenic species Methanosarcina acetivorans of the domain Archaea. Sequence analysis of MreA revealed identity to the TrmB family of...

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Main Authors:	Reichlen,, Matthew J., Vepachedu, Venkata R., Murakami, Katsuhiko S., Ferry, James G.
Format:	Online
Language:	English
Published:	American Society of Microbiology 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419521/

id	pubmed-3419521
recordtype	oai_dc
spelling	pubmed-34195212012-08-17 MreA Functions in the Global Regulation of Methanogenic Pathways in Methanosarcina acetivorans Reichlen,, Matthew J. Vepachedu, Venkata R. Murakami, Katsuhiko S. Ferry, James G. Research Article Results are presented supporting a regulatory role for the product of the MA3302 gene locus (designated MreA) previously annotated as a hypothetical protein in the methanogenic species Methanosarcina acetivorans of the domain Archaea. Sequence analysis of MreA revealed identity to the TrmB family of transcription factors, albeit the sequence is lacking the sensor domain analogous to TrmBL2, abundant in nonmethanogenic species of the domain Archaea. Transcription of mreA was highly upregulated during growth on acetate versus methylotrophic substrates, and an mreA deletion (ΔmreA) strain was impaired for growth with acetate in contrast to normal growth with methylotrophic substrates. Transcriptional profiling of acetate-grown cells identified 280 genes with altered expression in the ΔmreA strain versus the wild-type strain. Expression of genes unique to the acetate pathway decreased whereas expression of genes unique to methylotrophic metabolism increased in the ΔmreA strain relative to the wild type, results indicative of a dual role for MreA in either the direct or indirect activation of acetate-specific genes and repression of methylotrophic-specific genes. Gel shift experiments revealed specific binding of MreA to promoter regions of regulated genes. Homologs of MreA were identified in M. acetivorans and other Methanosarcina species for which expression patterns indicate roles in regulating methylotrophic pathways. American Society of Microbiology 2012-07-31 /pmc/articles/PMC3419521/ /pubmed/22851658 http://dx.doi.org/10.1128/mBio.00189-12 Text en Copyright © 2012 Reichlen et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Reichlen,, Matthew J. Vepachedu, Venkata R. Murakami, Katsuhiko S. Ferry, James G.
spellingShingle	Reichlen,, Matthew J. Vepachedu, Venkata R. Murakami, Katsuhiko S. Ferry, James G. MreA Functions in the Global Regulation of Methanogenic Pathways in Methanosarcina acetivorans
author_facet	Reichlen,, Matthew J. Vepachedu, Venkata R. Murakami, Katsuhiko S. Ferry, James G.
author_sort	Reichlen,, Matthew J.
title	MreA Functions in the Global Regulation of Methanogenic Pathways in Methanosarcina acetivorans
title_short	MreA Functions in the Global Regulation of Methanogenic Pathways in Methanosarcina acetivorans
title_full	MreA Functions in the Global Regulation of Methanogenic Pathways in Methanosarcina acetivorans
title_fullStr	MreA Functions in the Global Regulation of Methanogenic Pathways in Methanosarcina acetivorans
title_full_unstemmed	MreA Functions in the Global Regulation of Methanogenic Pathways in Methanosarcina acetivorans
title_sort	mrea functions in the global regulation of methanogenic pathways in methanosarcina acetivorans
description	Results are presented supporting a regulatory role for the product of the MA3302 gene locus (designated MreA) previously annotated as a hypothetical protein in the methanogenic species Methanosarcina acetivorans of the domain Archaea. Sequence analysis of MreA revealed identity to the TrmB family of transcription factors, albeit the sequence is lacking the sensor domain analogous to TrmBL2, abundant in nonmethanogenic species of the domain Archaea. Transcription of mreA was highly upregulated during growth on acetate versus methylotrophic substrates, and an mreA deletion (ΔmreA) strain was impaired for growth with acetate in contrast to normal growth with methylotrophic substrates. Transcriptional profiling of acetate-grown cells identified 280 genes with altered expression in the ΔmreA strain versus the wild-type strain. Expression of genes unique to the acetate pathway decreased whereas expression of genes unique to methylotrophic metabolism increased in the ΔmreA strain relative to the wild type, results indicative of a dual role for MreA in either the direct or indirect activation of acetate-specific genes and repression of methylotrophic-specific genes. Gel shift experiments revealed specific binding of MreA to promoter regions of regulated genes. Homologs of MreA were identified in M. acetivorans and other Methanosarcina species for which expression patterns indicate roles in regulating methylotrophic pathways.
publisher	American Society of Microbiology
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419521/
_version_	1611549812144996352

MreA Functions in the Global Regulation of Methanogenic Pathways in Methanosarcina acetivorans

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