Characterization of the Rate and Temperature Sensitivities of Bacterial Remineralization of Dissolved Organic Phosphorus Compounds by Natural Populations

Production, transformation, and degradation are the principal components of the cycling of dissolved organic matter (DOM) in marine systems. Heterotrophic Bacteria (and Archaea) play a large part in this cycling via enzymatic decomposition and intracellular transformations of organic material to ino...

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Main Authors:	White, Angelicque E., Watkins-Brandt, Katie S., Engle, Morgan A., Burkhardt, Brian, Paytan, Adina
Format:	Online
Language:	English
Published:	Frontiers Research Foundation 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3415674/

id	pubmed-3415674
recordtype	oai_dc
spelling	pubmed-34156742012-08-20 Characterization of the Rate and Temperature Sensitivities of Bacterial Remineralization of Dissolved Organic Phosphorus Compounds by Natural Populations White, Angelicque E. Watkins-Brandt, Katie S. Engle, Morgan A. Burkhardt, Brian Paytan, Adina Microbiology Production, transformation, and degradation are the principal components of the cycling of dissolved organic matter (DOM) in marine systems. Heterotrophic Bacteria (and Archaea) play a large part in this cycling via enzymatic decomposition and intracellular transformations of organic material to inorganic carbon (C), nitrogen (N), and phosphorus (P). The rate and magnitude of inorganic nutrient regeneration from DOM is related to the elemental composition and lability of DOM substrates as well as the nutritional needs of the mediating organisms. While many previous efforts have focused on C and N cycling of DOM, less is known in regards to the controls of organic P utilization and remineralization by natural populations of bacteria. In order to constrain the relative time scales and degradation of select dissolved organic P (DOP) compounds we have conducted a series of experiments focused on (1) assessment of the short-term lability of a range of DOP compounds, (2) characterization of labile DOP remineralization rates, and (3) examination of temperature sensitivities of labile DOP remineralization for varying bacterial populations. Results reinforce previous findings of monoester and polyphosphate lability and the relative recalcitrance of a model phosphonate: 2-aminoethylphosphonate. High resolution time-series of P-monoester remineralization indicates decay constants on the order of 0.67–7.04 day−1 for bacterial populations isolated from coastal and open ocean surface waters. The variability of these rates is predictably related to incubation temperature and initial concentrations of heterotrophic bacteria. Additional controls on DOP hydrolysis included seasonal shifts in bacterial populations and the physiological state of bacteria at the initiation of DOP addition experiments. Composite results indicate that bacterial hydrolysis of P-monoesters exceeds bacterial P demand and thus DOP remineralization efficiency may control P availability to autotrophs. Frontiers Research Foundation 2012-08-10 /pmc/articles/PMC3415674/ /pubmed/22908008 http://dx.doi.org/10.3389/fmicb.2012.00276 Text en Copyright © 2012 White, Watkins-Brandt, Engle, Burkhardt and Paytan. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	White, Angelicque E. Watkins-Brandt, Katie S. Engle, Morgan A. Burkhardt, Brian Paytan, Adina
spellingShingle	White, Angelicque E. Watkins-Brandt, Katie S. Engle, Morgan A. Burkhardt, Brian Paytan, Adina Characterization of the Rate and Temperature Sensitivities of Bacterial Remineralization of Dissolved Organic Phosphorus Compounds by Natural Populations
author_facet	White, Angelicque E. Watkins-Brandt, Katie S. Engle, Morgan A. Burkhardt, Brian Paytan, Adina
author_sort	White, Angelicque E.
title	Characterization of the Rate and Temperature Sensitivities of Bacterial Remineralization of Dissolved Organic Phosphorus Compounds by Natural Populations
title_short	Characterization of the Rate and Temperature Sensitivities of Bacterial Remineralization of Dissolved Organic Phosphorus Compounds by Natural Populations
title_full	Characterization of the Rate and Temperature Sensitivities of Bacterial Remineralization of Dissolved Organic Phosphorus Compounds by Natural Populations
title_fullStr	Characterization of the Rate and Temperature Sensitivities of Bacterial Remineralization of Dissolved Organic Phosphorus Compounds by Natural Populations
title_full_unstemmed	Characterization of the Rate and Temperature Sensitivities of Bacterial Remineralization of Dissolved Organic Phosphorus Compounds by Natural Populations
title_sort	characterization of the rate and temperature sensitivities of bacterial remineralization of dissolved organic phosphorus compounds by natural populations
description	Production, transformation, and degradation are the principal components of the cycling of dissolved organic matter (DOM) in marine systems. Heterotrophic Bacteria (and Archaea) play a large part in this cycling via enzymatic decomposition and intracellular transformations of organic material to inorganic carbon (C), nitrogen (N), and phosphorus (P). The rate and magnitude of inorganic nutrient regeneration from DOM is related to the elemental composition and lability of DOM substrates as well as the nutritional needs of the mediating organisms. While many previous efforts have focused on C and N cycling of DOM, less is known in regards to the controls of organic P utilization and remineralization by natural populations of bacteria. In order to constrain the relative time scales and degradation of select dissolved organic P (DOP) compounds we have conducted a series of experiments focused on (1) assessment of the short-term lability of a range of DOP compounds, (2) characterization of labile DOP remineralization rates, and (3) examination of temperature sensitivities of labile DOP remineralization for varying bacterial populations. Results reinforce previous findings of monoester and polyphosphate lability and the relative recalcitrance of a model phosphonate: 2-aminoethylphosphonate. High resolution time-series of P-monoester remineralization indicates decay constants on the order of 0.67–7.04 day−1 for bacterial populations isolated from coastal and open ocean surface waters. The variability of these rates is predictably related to incubation temperature and initial concentrations of heterotrophic bacteria. Additional controls on DOP hydrolysis included seasonal shifts in bacterial populations and the physiological state of bacteria at the initiation of DOP addition experiments. Composite results indicate that bacterial hydrolysis of P-monoesters exceeds bacterial P demand and thus DOP remineralization efficiency may control P availability to autotrophs.
publisher	Frontiers Research Foundation
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3415674/
_version_	1611548871044890624

Characterization of the Rate and Temperature Sensitivities of Bacterial Remineralization of Dissolved Organic Phosphorus Compounds by Natural Populations

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