Time-resolved protein nanocrystallography using an X-ray free-electron laser

We demonstrate the use of an X-ray free electron laser synchronized with an optical pump laser to obtain X-ray diffraction snapshots from the photoactivated states of large membrane protein complexes in the form of nanocrystals flowing in a liquid jet. Light-induced changes of Photosystem I-Ferredox...

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Bibliographic Details
Main Authors: Aquila, Andrew, Hunter, Mark S., Doak, R. Bruce, Kirian, Richard A., Fromme, Petra, White, Thomas A., Andreasson, Jakob, Arnlund, David, Bajt, Saša, Barends, Thomas R. M., Barthelmess, Miriam, Bogan, Michael J., Bostedt, Christoph, Bottin, Hervé, Bozek, John D., Caleman, Carl, Coppola, Nicola, Davidsson, Jan, DePonte, Daniel P., Elser, Veit, Epp, Sascha W., Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Frank, Matthias, Fromme, Raimund, Graafsma, Heinz, Grotjohann, Ingo, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y., Hartmann, Andreas, Hartmann, Robert, Hau-Riege, Stefan, Hauser, Günter, Hirsemann, Helmut, Holl, Peter, Holton, James M., Hömke, André, Johansson, Linda, Kimmel, Nils, Kassemeyer, Stephan, Krasniqi, Faton, Kühnel, Kai-Uwe, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Marchesini, Stefano, Martin, Andrew V., Maia, Filipe R.N.C., Messerschmidt, Marc, Nass, Karol, Reich, Christian, Neutze, Richard, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schlichting, Ilme, Schmidt, Carlo, Schmidt, Kevin E., Schulz, Joachim, Seibert, M. Marvin, Shoeman, Robert L., Sierra, Raymond, Soltau, Heike, Starodub, Dmitri, Stellato, Francesco, Stern, Stephan, Strüder, Lothar, Timneanu, Nicusor, Ullrich, Joachim, Wang, Xiaoyu, Williams, Garth J., Weidenspointner, Georg, Weierstall, Uwe, Wunderer, Cornelia, Barty, Anton, Spence, John C. H., Chapman, Henry N.
Format: Online
Language:English
Published: Optical Society of America 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413412/