Development of EST-SSR markers of Ipomoea nil

Development of EST-SSR markers of Ipomoea nil

Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for futur...

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Main Authors: Ly, Tong, Fukuoka, Hiroyuki, Otaka, Asami, Hoshino, Atsushi, Iida, Shigeru, Nitasaka, Eiji, Watanabe, Nobuyoshi, Kuboyama, Tsutomu
Format: Online
Language:English
Published: Japanese Society of Breeding 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405949/
id pubmed-3405949
recordtype oai_dc
spelling pubmed-34059492012-11-07 Development of EST-SSR markers of Ipomoea nil Ly, Tong Fukuoka, Hiroyuki Otaka, Asami Hoshino, Atsushi Iida, Shigeru Nitasaka, Eiji Watanabe, Nobuyoshi Kuboyama, Tsutomu Note Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives. Japanese Society of Breeding 2012-03-20 2012-03 /pmc/articles/PMC3405949/ /pubmed/23136520 http://dx.doi.org/10.1270/jsbbs.62.99 Text en Copyright © 2012 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Ly, Tong
Fukuoka, Hiroyuki
Otaka, Asami
Hoshino, Atsushi
Iida, Shigeru
Nitasaka, Eiji
Watanabe, Nobuyoshi
Kuboyama, Tsutomu
spellingShingle Ly, Tong
Fukuoka, Hiroyuki
Otaka, Asami
Hoshino, Atsushi
Iida, Shigeru
Nitasaka, Eiji
Watanabe, Nobuyoshi
Kuboyama, Tsutomu
Development of EST-SSR markers of Ipomoea nil
author_facet Ly, Tong
Fukuoka, Hiroyuki
Otaka, Asami
Hoshino, Atsushi
Iida, Shigeru
Nitasaka, Eiji
Watanabe, Nobuyoshi
Kuboyama, Tsutomu
author_sort Ly, Tong
title Development of EST-SSR markers of Ipomoea nil
title_short Development of EST-SSR markers of Ipomoea nil
title_full Development of EST-SSR markers of Ipomoea nil
title_fullStr Development of EST-SSR markers of Ipomoea nil
title_full_unstemmed Development of EST-SSR markers of Ipomoea nil
title_sort development of est-ssr markers of ipomoea nil
description Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives.
publisher Japanese Society of Breeding
publishDate 2012
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405949/
_version_ 1611545787866546176

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