Identification and Functional Analysis of Three Isoforms of Bovine BST-2

Human BST-2 (hBST-2) has been identified as a cellular antiviral factor that blocks the release of various enveloped viruses. Orthologues of BST-2 have been identified in several species, including human, monkeys, pig, mouse, cat and sheep. All have been reported to possess antiviral activity. Dupli...

Full description

Bibliographic Details
Main Authors:	Takeda, Eri, Nakagawa, So, Nakaya, Yuki, Tanaka, Atsushi, Miyazawa, Takayuki, Yasuda, Jiro
Format:	Online
Language:	English
Published:	Public Library of Science 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401110/

id	pubmed-3401110
recordtype	oai_dc
spelling	pubmed-34011102012-07-30 Identification and Functional Analysis of Three Isoforms of Bovine BST-2 Takeda, Eri Nakagawa, So Nakaya, Yuki Tanaka, Atsushi Miyazawa, Takayuki Yasuda, Jiro Research Article Human BST-2 (hBST-2) has been identified as a cellular antiviral factor that blocks the release of various enveloped viruses. Orthologues of BST-2 have been identified in several species, including human, monkeys, pig, mouse, cat and sheep. All have been reported to possess antiviral activity. Duplication of the BST-2 gene has been observed in sheep and the paralogues are referred to as ovine BST-2A and BST2-B, although only a single gene corresponding to BST-2 has been identified in most species. In this study, we identified three isoforms of bovine BST-2, named bBST-2A1, bBST-2A2 and bBST-2B, in bovine cells treated with type I interferon, but not in untreated cells. Both bBST-2A1 and bBST-2A2 are posttranslationally modified by N-linked glycosylation and a GPI-anchor as well as hBST-2, while bBST-2B has neither of these modifications. Exogenous expression of bBST-2A1 or bBST-2A2 markedly reduced the production of bovine leukemia virus and vesicular stomatitis virus from cells, while the antiviral activity of bBST-2B was much weaker than those of bBST-2A1 and bBST-2A2. Our data suggest that bBST-2A1 and bBST-2A2 function as part of IFN-induced innate immunity against virus infection. On the other hand, bBST-2B may have a different physiological function from bBST-2A1 and bBST-2A2. Public Library of Science 2012-07-20 /pmc/articles/PMC3401110/ /pubmed/22911799 http://dx.doi.org/10.1371/journal.pone.0041483 Text en Takeda et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Takeda, Eri Nakagawa, So Nakaya, Yuki Tanaka, Atsushi Miyazawa, Takayuki Yasuda, Jiro
spellingShingle	Takeda, Eri Nakagawa, So Nakaya, Yuki Tanaka, Atsushi Miyazawa, Takayuki Yasuda, Jiro Identification and Functional Analysis of Three Isoforms of Bovine BST-2
author_facet	Takeda, Eri Nakagawa, So Nakaya, Yuki Tanaka, Atsushi Miyazawa, Takayuki Yasuda, Jiro
author_sort	Takeda, Eri
title	Identification and Functional Analysis of Three Isoforms of Bovine BST-2
title_short	Identification and Functional Analysis of Three Isoforms of Bovine BST-2
title_full	Identification and Functional Analysis of Three Isoforms of Bovine BST-2
title_fullStr	Identification and Functional Analysis of Three Isoforms of Bovine BST-2
title_full_unstemmed	Identification and Functional Analysis of Three Isoforms of Bovine BST-2
title_sort	identification and functional analysis of three isoforms of bovine bst-2
description	Human BST-2 (hBST-2) has been identified as a cellular antiviral factor that blocks the release of various enveloped viruses. Orthologues of BST-2 have been identified in several species, including human, monkeys, pig, mouse, cat and sheep. All have been reported to possess antiviral activity. Duplication of the BST-2 gene has been observed in sheep and the paralogues are referred to as ovine BST-2A and BST2-B, although only a single gene corresponding to BST-2 has been identified in most species. In this study, we identified three isoforms of bovine BST-2, named bBST-2A1, bBST-2A2 and bBST-2B, in bovine cells treated with type I interferon, but not in untreated cells. Both bBST-2A1 and bBST-2A2 are posttranslationally modified by N-linked glycosylation and a GPI-anchor as well as hBST-2, while bBST-2B has neither of these modifications. Exogenous expression of bBST-2A1 or bBST-2A2 markedly reduced the production of bovine leukemia virus and vesicular stomatitis virus from cells, while the antiviral activity of bBST-2B was much weaker than those of bBST-2A1 and bBST-2A2. Our data suggest that bBST-2A1 and bBST-2A2 function as part of IFN-induced innate immunity against virus infection. On the other hand, bBST-2B may have a different physiological function from bBST-2A1 and bBST-2A2.
publisher	Public Library of Science
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401110/
_version_	1611544217958481920

Identification and Functional Analysis of Three Isoforms of Bovine BST-2

Similar Items