Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles

We present the implementation of a combined digital scanned light-sheet microscope (DSLM) able to work in the linear and nonlinear regimes under either Gaussian or Bessel beam excitation schemes. A complete characterization of the setup is performed and a comparison of the performance of each DSLM i...

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Main Authors:	Olarte, Omar E., Licea-Rodriguez, Jacob, Palero, Jonathan A., Gualda, Emilio J., Artigas, David, Mayer, Jürgen, Swoger, Jim, Sharpe, James, Rocha-Mendoza, Israel, Rangel-Rojo, Raul, Loza-Alvarez, Pablo
Format:	Online
Language:	English
Published:	Optical Society of America 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395476/

id	pubmed-3395476
recordtype	oai_dc
spelling	pubmed-33954762012-07-17 Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles Olarte, Omar E. Licea-Rodriguez, Jacob Palero, Jonathan A. Gualda, Emilio J. Artigas, David Mayer, Jürgen Swoger, Jim Sharpe, James Rocha-Mendoza, Israel Rangel-Rojo, Raul Loza-Alvarez, Pablo Microscopy We present the implementation of a combined digital scanned light-sheet microscope (DSLM) able to work in the linear and nonlinear regimes under either Gaussian or Bessel beam excitation schemes. A complete characterization of the setup is performed and a comparison of the performance of each DSLM imaging modality is presented using in vivo Caenorhabditis elegans samples. We found that the use of Bessel beam nonlinear excitation results in better image contrast over a wider field of view. Optical Society of America 2012-06-04 /pmc/articles/PMC3395476/ /pubmed/22808423 http://dx.doi.org/10.1364/BOE.3.001492 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Olarte, Omar E. Licea-Rodriguez, Jacob Palero, Jonathan A. Gualda, Emilio J. Artigas, David Mayer, Jürgen Swoger, Jim Sharpe, James Rocha-Mendoza, Israel Rangel-Rojo, Raul Loza-Alvarez, Pablo
spellingShingle	Olarte, Omar E. Licea-Rodriguez, Jacob Palero, Jonathan A. Gualda, Emilio J. Artigas, David Mayer, Jürgen Swoger, Jim Sharpe, James Rocha-Mendoza, Israel Rangel-Rojo, Raul Loza-Alvarez, Pablo Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles
author_facet	Olarte, Omar E. Licea-Rodriguez, Jacob Palero, Jonathan A. Gualda, Emilio J. Artigas, David Mayer, Jürgen Swoger, Jim Sharpe, James Rocha-Mendoza, Israel Rangel-Rojo, Raul Loza-Alvarez, Pablo
author_sort	Olarte, Omar E.
title	Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles
title_short	Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles
title_full	Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles
title_fullStr	Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles
title_full_unstemmed	Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles
title_sort	image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with gaussian and bessel beam profiles
description	We present the implementation of a combined digital scanned light-sheet microscope (DSLM) able to work in the linear and nonlinear regimes under either Gaussian or Bessel beam excitation schemes. A complete characterization of the setup is performed and a comparison of the performance of each DSLM imaging modality is presented using in vivo Caenorhabditis elegans samples. We found that the use of Bessel beam nonlinear excitation results in better image contrast over a wider field of view.
publisher	Optical Society of America
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395476/
_version_	1611542723692593152

Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles

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