The Inhibition of Anti-DNA Binding to DNA by Nucleic Acid Binding Polymers

The Inhibition of Anti-DNA Binding to DNA by Nucleic Acid Binding Polymers

Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus (SLE) and can mediate disease pathogenesis by the formation of immune complexes. Since blocking immune complex formation can attenuate disease manifestations, the effects of nucleic acid binding polymers (NABPs...

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Main Authors: Stearns, Nancy A., Lee, Jaewoo, Leong, Kam W., Sullenger, Bruce A., Pisetsky, David S.
Format: Online
Language:English
Published: Public Library of Science 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3394750/
id pubmed-3394750
recordtype oai_dc
spelling pubmed-33947502012-07-17 The Inhibition of Anti-DNA Binding to DNA by Nucleic Acid Binding Polymers Stearns, Nancy A. Lee, Jaewoo Leong, Kam W. Sullenger, Bruce A. Pisetsky, David S. Research Article Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus (SLE) and can mediate disease pathogenesis by the formation of immune complexes. Since blocking immune complex formation can attenuate disease manifestations, the effects of nucleic acid binding polymers (NABPs) on anti-DNA binding in vitro were investigated. The compounds tested included polyamidoamine dendrimer, 1,4-diaminobutane core, generation 3.0 (PAMAM-G3), hexadimethrine bromide, and a β-cylodextrin-containing polycation. As shown with plasma from patients with SLE, NABPs can inhibit anti-DNA antibody binding in ELISA assays. The inhibition was specific since the NABPs did not affect binding to tetanus toxoid or the Sm protein, another lupus autoantigen. Furthermore, the polymers could displace antibody from preformed complexes. Together, these results indicate that NABPs can inhibit the formation of immune complexes and may represent a new approach to treatment. Public Library of Science 2012-07-11 /pmc/articles/PMC3394750/ /pubmed/22808279 http://dx.doi.org/10.1371/journal.pone.0040862 Text en Stearns et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Stearns, Nancy A.
Lee, Jaewoo
Leong, Kam W.
Sullenger, Bruce A.
Pisetsky, David S.
spellingShingle Stearns, Nancy A.
Lee, Jaewoo
Leong, Kam W.
Sullenger, Bruce A.
Pisetsky, David S.
The Inhibition of Anti-DNA Binding to DNA by Nucleic Acid Binding Polymers
author_facet Stearns, Nancy A.
Lee, Jaewoo
Leong, Kam W.
Sullenger, Bruce A.
Pisetsky, David S.
author_sort Stearns, Nancy A.
title The Inhibition of Anti-DNA Binding to DNA by Nucleic Acid Binding Polymers
title_short The Inhibition of Anti-DNA Binding to DNA by Nucleic Acid Binding Polymers
title_full The Inhibition of Anti-DNA Binding to DNA by Nucleic Acid Binding Polymers
title_fullStr The Inhibition of Anti-DNA Binding to DNA by Nucleic Acid Binding Polymers
title_full_unstemmed The Inhibition of Anti-DNA Binding to DNA by Nucleic Acid Binding Polymers
title_sort inhibition of anti-dna binding to dna by nucleic acid binding polymers
description Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus (SLE) and can mediate disease pathogenesis by the formation of immune complexes. Since blocking immune complex formation can attenuate disease manifestations, the effects of nucleic acid binding polymers (NABPs) on anti-DNA binding in vitro were investigated. The compounds tested included polyamidoamine dendrimer, 1,4-diaminobutane core, generation 3.0 (PAMAM-G3), hexadimethrine bromide, and a β-cylodextrin-containing polycation. As shown with plasma from patients with SLE, NABPs can inhibit anti-DNA antibody binding in ELISA assays. The inhibition was specific since the NABPs did not affect binding to tetanus toxoid or the Sm protein, another lupus autoantigen. Furthermore, the polymers could displace antibody from preformed complexes. Together, these results indicate that NABPs can inhibit the formation of immune complexes and may represent a new approach to treatment.
publisher Public Library of Science
publishDate 2012
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3394750/
_version_ 1611542474917937152

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