Quality-Score Refinement of SSU rRNA Gene Pyrosequencing Differs Across Gene Region for Environmental Samples
Due to potential sequencing errors in pyrosequencing data, species richness and diversity indices of microbial systems can be miscalculated. The “traditional” sequence refinement method is not sufficient to account for overestimations (e.g., length, primer errors, ambiguous nucleotides). Recent in s...
| Main Authors: | , , |
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| Format: | Online |
| Language: | English |
| Published: |
Springer-Verlag
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391548/ |