MicroRNA 107 Partly Inhibits Endothelial Progenitor Cells Differentiation via HIF-1β

Endothelial progenitor cells (EPCs) play an important role in tissue repair after ischemic heart disease. In particular, the recovery of endothelial function is reliant on the ability and rate of EPCs differentiate into mature endothelial cells. The present study evaluated the effect of microRNA 107...

Full description

Bibliographic Details
Main Authors:	Meng, Shu, Cao, JiaTian, Wang, LianSheng, Zhou, Qing, Li, YiGang, Shen, ChengXing, Zhang, XiaoPing, Wang, ChangQian
Format:	Online
Language:	English
Published:	Public Library of Science 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391260/

id	pubmed-3391260
recordtype	oai_dc
spelling	pubmed-33912602012-07-12 MicroRNA 107 Partly Inhibits Endothelial Progenitor Cells Differentiation via HIF-1β Meng, Shu Cao, JiaTian Wang, LianSheng Zhou, Qing Li, YiGang Shen, ChengXing Zhang, XiaoPing Wang, ChangQian Research Article Endothelial progenitor cells (EPCs) play an important role in tissue repair after ischemic heart disease. In particular, the recovery of endothelial function is reliant on the ability and rate of EPCs differentiate into mature endothelial cells. The present study evaluated the effect of microRNA 107 (miR-107) on the mechanism of EPCs differentiation. EPCs were isolated from rats' bone marrow and miR-107 expression of EPCs in hypoxic and normoxic conditions were measured by real-time qualitative PCR. CD31 was analyzed by flow cytometry and eNOS was examined by real-time qualitative PCR and western blotting and these were used as markers of EPC differentiation. In order to reveal the mechanism, we used miR107 inhibitor and lentiviral vector expressing a short hairpin RNA (shRNA) that targets miR-107 and hypoxia-inducible factor-1 β (HIF-1β) to alter miR107 and HIF-1β expression. MiR-107 expression were increased in EPCs under hypoxic conditions. Up-regulation of miR-107 partly suppressed the EPCs differentiation induced in hypoxia, while down-regulation of miR-107 promoted EPC differentiation. HIF-1β was the target. This study indicated that miR-107 was up-regulated in hypoxia to prevent EPCs differentiation via its target HIF-1β. The physiological mechanisms of miR-107 must be evaluated if it is to be used as a potential anti-ischemia therapeutic regime. Public Library of Science 2012-07-06 /pmc/articles/PMC3391260/ /pubmed/22792280 http://dx.doi.org/10.1371/journal.pone.0040323 Text en Meng et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Meng, Shu Cao, JiaTian Wang, LianSheng Zhou, Qing Li, YiGang Shen, ChengXing Zhang, XiaoPing Wang, ChangQian
spellingShingle	Meng, Shu Cao, JiaTian Wang, LianSheng Zhou, Qing Li, YiGang Shen, ChengXing Zhang, XiaoPing Wang, ChangQian MicroRNA 107 Partly Inhibits Endothelial Progenitor Cells Differentiation via HIF-1β
author_facet	Meng, Shu Cao, JiaTian Wang, LianSheng Zhou, Qing Li, YiGang Shen, ChengXing Zhang, XiaoPing Wang, ChangQian
author_sort	Meng, Shu
title	MicroRNA 107 Partly Inhibits Endothelial Progenitor Cells Differentiation via HIF-1β
title_short	MicroRNA 107 Partly Inhibits Endothelial Progenitor Cells Differentiation via HIF-1β
title_full	MicroRNA 107 Partly Inhibits Endothelial Progenitor Cells Differentiation via HIF-1β
title_fullStr	MicroRNA 107 Partly Inhibits Endothelial Progenitor Cells Differentiation via HIF-1β
title_full_unstemmed	MicroRNA 107 Partly Inhibits Endothelial Progenitor Cells Differentiation via HIF-1β
title_sort	microrna 107 partly inhibits endothelial progenitor cells differentiation via hif-1β
description	Endothelial progenitor cells (EPCs) play an important role in tissue repair after ischemic heart disease. In particular, the recovery of endothelial function is reliant on the ability and rate of EPCs differentiate into mature endothelial cells. The present study evaluated the effect of microRNA 107 (miR-107) on the mechanism of EPCs differentiation. EPCs were isolated from rats' bone marrow and miR-107 expression of EPCs in hypoxic and normoxic conditions were measured by real-time qualitative PCR. CD31 was analyzed by flow cytometry and eNOS was examined by real-time qualitative PCR and western blotting and these were used as markers of EPC differentiation. In order to reveal the mechanism, we used miR107 inhibitor and lentiviral vector expressing a short hairpin RNA (shRNA) that targets miR-107 and hypoxia-inducible factor-1 β (HIF-1β) to alter miR107 and HIF-1β expression. MiR-107 expression were increased in EPCs under hypoxic conditions. Up-regulation of miR-107 partly suppressed the EPCs differentiation induced in hypoxia, while down-regulation of miR-107 promoted EPC differentiation. HIF-1β was the target. This study indicated that miR-107 was up-regulated in hypoxia to prevent EPCs differentiation via its target HIF-1β. The physiological mechanisms of miR-107 must be evaluated if it is to be used as a potential anti-ischemia therapeutic regime.
publisher	Public Library of Science
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391260/
_version_	1611541475982573568

MicroRNA 107 Partly Inhibits Endothelial Progenitor Cells Differentiation via HIF-1β

Similar Items