The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest
In this work, the toxicity and genotoxicity of organic solvents (acetone, carbon tetrachloride, dichloromethane, dimethylsulfoxide, ethanol, ether and methanol) were studied using the SOS chromotest. The influence of these solvents on the direct genotoxicity induced by the mutagens mitomycin C (MMC)...
| Main Authors: | , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Sociedade Brasileira de Genética
2012
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3389540/ |
| id |
pubmed-3389540 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-33895402012-08-10 The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest Quintero, Nathalia Stashenko, Elena E. Fuentes, Jorge Luis Mutagenesis In this work, the toxicity and genotoxicity of organic solvents (acetone, carbon tetrachloride, dichloromethane, dimethylsulfoxide, ethanol, ether and methanol) were studied using the SOS chromotest. The influence of these solvents on the direct genotoxicity induced by the mutagens mitomycin C (MMC) and 4-nitroquinoline-1-oxide (4-NQO) were also investigated. None of the solvents were genotoxic in Escherichia coli PQ37. However, based on the inhibition of protein synthesis assessed by constitutive alkaline phosphatase activity, some solvents (carbon tetrachloride, dimethylsulfoxide, ethanol and ether) were toxic and incompatible with the SOS chromotest. Solvents that were neither toxic nor genotoxic to E. coli (acetone, dichloromethane and methanol) significantly reduced the genotoxicity of MMC and 4-NQO. When these solvents were used to dissolve vitamin E they increased the antigenotoxic activity of this compound, possibly through additive or synergistic effects. The relevance of these results is discussed in relation to antigenotoxic studies. These data indicate the need for careful selection of an appropriate diluent for the SOS chromotest since some solvents can modulate genotoxicity and antigenotoxicity. Sociedade Brasileira de Genética 2012 2012-06-23 /pmc/articles/PMC3389540/ /pubmed/22888301 http://dx.doi.org/10.1590/S1415-47572012000300018 Text en Copyright © 2012, Sociedade Brasileira de Genética. License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Quintero, Nathalia Stashenko, Elena E. Fuentes, Jorge Luis |
| spellingShingle |
Quintero, Nathalia Stashenko, Elena E. Fuentes, Jorge Luis The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest |
| author_facet |
Quintero, Nathalia Stashenko, Elena E. Fuentes, Jorge Luis |
| author_sort |
Quintero, Nathalia |
| title |
The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest |
| title_short |
The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest |
| title_full |
The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest |
| title_fullStr |
The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest |
| title_full_unstemmed |
The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest |
| title_sort |
influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the sos chromotest |
| description |
In this work, the toxicity and genotoxicity of organic solvents (acetone, carbon tetrachloride, dichloromethane, dimethylsulfoxide, ethanol, ether and methanol) were studied using the SOS chromotest. The influence of these solvents on the direct genotoxicity induced by the mutagens mitomycin C (MMC) and 4-nitroquinoline-1-oxide (4-NQO) were also investigated. None of the solvents were genotoxic in Escherichia coli PQ37. However, based on the inhibition of protein synthesis assessed by constitutive alkaline phosphatase activity, some solvents (carbon tetrachloride, dimethylsulfoxide, ethanol and ether) were toxic and incompatible with the SOS chromotest. Solvents that were neither toxic nor genotoxic to E. coli (acetone, dichloromethane and methanol) significantly reduced the genotoxicity of MMC and 4-NQO. When these solvents were used to dissolve vitamin E they increased the antigenotoxic activity of this compound, possibly through additive or synergistic effects. The relevance of these results is discussed in relation to antigenotoxic studies. These data indicate the need for careful selection of an appropriate diluent for the SOS chromotest since some solvents can modulate genotoxicity and antigenotoxicity. |
| publisher |
Sociedade Brasileira de Genética |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3389540/ |
| _version_ |
1611541016374935552 |