Galectin-3 increases the motility of mouse melanoma cells by regulating matrix metalloproteinase-1 expression
Although mounting evidence indicates the involvement of galectin-3 in cancer progression and metastasis, the underlying molecular mechanisms remain largely unknown. In this study, we investigated the effect and possible mechanism of galectin-3 on the migration and invasion of B16F10, a metastatic me...
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Korean Society for Biochemistry and Molecular Biology
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3389077/ |
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pubmed-33890772012-07-11 Galectin-3 increases the motility of mouse melanoma cells by regulating matrix metalloproteinase-1 expression Wang, Yuan-Guo Kim, Seok-Jun Baek, Jung-Hwan Lee, Hyun-Woo Jeong, Seo-Young Chun, Kyung-Hee Original Article Although mounting evidence indicates the involvement of galectin-3 in cancer progression and metastasis, the underlying molecular mechanisms remain largely unknown. In this study, we investigated the effect and possible mechanism of galectin-3 on the migration and invasion of B16F10, a metastatic melanoma cell line, in which galectin-3 and matrix metalloproteinase-1 (MMP-1) were both found to be highly expressed. Knockdown of galectin-3 with specific siRNA reduced migration and invasion, which was associated with reduced expression of MMP-1. To further investigate the underlying mechanism, we examined the effect of galectin-3 knockdown on the activity of AP-1, a transcriptional factor regulating MMP-1 expression. We found that galectin-3 directly interacted with AP-1 and facilitated the binding of this complex to the MMP-1 promoter that drives MMP-1 transcription. Moreover, silencing of galectin-3 inhibited binding of fra-1 and c-Jun to promoter sites of MMP-1 gene. Consistent with these in vitro findings, our in vivo study demonstrated that galectin-3 shRNA treatment significantly reduced the total number of mouse lung metastatic nodules. Taken together, galectin-3 facilitates cell migration and invasion in melanoma in vitro and can induce metastasis in vivo, in part through, regulating the transcription activity of AP-1 and thereby up-regulating MMP-1 expression. Korean Society for Biochemistry and Molecular Biology 2012-06-30 2012-03-21 /pmc/articles/PMC3389077/ /pubmed/22437631 http://dx.doi.org/10.3858/emm.2012.44.6.044 Text en Copyright © 2012 by the Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Wang, Yuan-Guo Kim, Seok-Jun Baek, Jung-Hwan Lee, Hyun-Woo Jeong, Seo-Young Chun, Kyung-Hee |
| spellingShingle |
Wang, Yuan-Guo Kim, Seok-Jun Baek, Jung-Hwan Lee, Hyun-Woo Jeong, Seo-Young Chun, Kyung-Hee Galectin-3 increases the motility of mouse melanoma cells by regulating matrix metalloproteinase-1 expression |
| author_facet |
Wang, Yuan-Guo Kim, Seok-Jun Baek, Jung-Hwan Lee, Hyun-Woo Jeong, Seo-Young Chun, Kyung-Hee |
| author_sort |
Wang, Yuan-Guo |
| title |
Galectin-3 increases the motility of mouse melanoma cells by regulating matrix metalloproteinase-1 expression |
| title_short |
Galectin-3 increases the motility of mouse melanoma cells by regulating matrix metalloproteinase-1 expression |
| title_full |
Galectin-3 increases the motility of mouse melanoma cells by regulating matrix metalloproteinase-1 expression |
| title_fullStr |
Galectin-3 increases the motility of mouse melanoma cells by regulating matrix metalloproteinase-1 expression |
| title_full_unstemmed |
Galectin-3 increases the motility of mouse melanoma cells by regulating matrix metalloproteinase-1 expression |
| title_sort |
galectin-3 increases the motility of mouse melanoma cells by regulating matrix metalloproteinase-1 expression |
| description |
Although mounting evidence indicates the involvement of galectin-3 in cancer progression and metastasis, the underlying molecular mechanisms remain largely unknown. In this study, we investigated the effect and possible mechanism of galectin-3 on the migration and invasion of B16F10, a metastatic melanoma cell line, in which galectin-3 and matrix metalloproteinase-1 (MMP-1) were both found to be highly expressed. Knockdown of galectin-3 with specific siRNA reduced migration and invasion, which was associated with reduced expression of MMP-1. To further investigate the underlying mechanism, we examined the effect of galectin-3 knockdown on the activity of AP-1, a transcriptional factor regulating MMP-1 expression. We found that galectin-3 directly interacted with AP-1 and facilitated the binding of this complex to the MMP-1 promoter that drives MMP-1 transcription. Moreover, silencing of galectin-3 inhibited binding of fra-1 and c-Jun to promoter sites of MMP-1 gene. Consistent with these in vitro findings, our in vivo study demonstrated that galectin-3 shRNA treatment significantly reduced the total number of mouse lung metastatic nodules. Taken together, galectin-3 facilitates cell migration and invasion in melanoma in vitro and can induce metastasis in vivo, in part through, regulating the transcription activity of AP-1 and thereby up-regulating MMP-1 expression. |
| publisher |
Korean Society for Biochemistry and Molecular Biology |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3389077/ |
| _version_ |
1611540898328346624 |